CarrierMax™ FMR1 Reagent Kit - FAQs

We recommend using the following controls for CarrierMax FMR1 Reagent Kit:
- NA20236: FMR1 gene (Not affected) from Coriell Institute
- NA06968: FMR1 gene (Premutation/Carrier) from Coriell Institute
- NA06897: FMR1 gene (Affected) from Coriell Institute
- NA23687: SMA1 gene (Carrier) from Coriell Institute
- NA00232: SMA1 gene (Homozygote affected) from Coriell Institute
- NA04408: SMA1 gene (Risk factor (Pathogenic SNP)) from Coriell Institute
- NA11859: (SMA1) gene (Normal) from Coriell Institute

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

We have GeneMapper analysis parameters for the CarrierMax kits, specifically for the SeqStudio and the 3500/3500xL instruments. They can be found on the following links or the CarrierMax kit product page. http://downloads.thermofisher.com/GeneMapper/FMR1_SeqStudio_GeneMapper_files.zip http://downloads.thermofisher.com/GeneMapper/FMR1_3500_GeneMapper_files.zip

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

To analyze data from the CarrierMax FMR1 Reagent Kit, you would have to use both the GeneMapper Software and CarrierMax Software for genotyping and classification of the sample, respectively.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

The QD1200 size standard found in the CarrierMax FMR1 Reagent Kit is not available separately.

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The CarrierMax FMR1 Reagent Kit uses the QD1200 size standard.

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The CarrierMax FMR1 Reagent Kit uses 6-FAM fluorescent dye label for the primers and AF633 fluorescent dye label for the size standard.

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The CarrierMax kits have been tested on the Veriti Thermal Cycler, ProFlex 96‑well PCR System, and GeneAmp PCR System 9700.

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For the CarrierMax FMR1 Reagent Kit, we recommend using 40 ng of gDNA for each PCR reaction.

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We recommend using purified gDNA template with the CarrierMax FMR1 Reagent Kit.

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With the CarrierMax FMR1 Reagent Kit, two primer sets are used, with one set designed to amplify the full length (FL) of CGG expansion and the second set designed to amplify within the CGG repeat (RP). The results from both reactions are used in carrier status classification.

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The CarrierMax FMR1 Reagent Kit contains the following components:
- CarrierMax FMR1 Reagent PCR Reaction Buffer
- CarrierMax FMR1 Reagent Enzyme Mix
- CarrierMax FMR1 Reagent Full Length Primers Mix
- CarrierMax FMR1 Reagent Repeat Primers Mix
- CarrierMax FMR1 Reagent Nuclease-free water
- CarrierMax FMR1 Reagent QD1200 Size Standard

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

The CarrierMax FMR1 Reagent Kit has been tested for use on the Applied Biosystems 3500 Genetic Analyzer, Applied Biosystems 3500xL Genetic Analyzer, and Applied Biosystems SeqStudio Genetic Analyzer.

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48 samples can be processed with the CarrierMax FMR1 Reagent Kit. Please note that each sample will require 2 PCR reactions, for a total of 96 PCR reactions per kit.

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The CarrierMax A5D Matrix Standard Kit contains 1 tube of matrix standard, sufficient for a minimum of 8 runs.

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The CarrierMax A5D Matrix Standard Kit should not be frozen/thawed more than 5 times.

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We recommend storing the CarrierMax A5D Matrix Standard Kit at -25 degrees C to -15 degrees C, protected from light.

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The matrix standard in the CarrierMax A5D Matrix Standard Kit contains five DNA fragments, each size labeled with a different dye from the dye set of five fluorescent dyes: 6-FAM dye, HEX dye, TAMRA dye, ROX dye, and AF633 dye.

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The CarrierMax A5D Matrix Standard Kit has been tested for use on the Applied Biosystems 3500 Genetic Analyzer, Applied Biosystems 3500xL Genetic Analyzer, and Applied Biosystems SeqStudio Genetic Analyzer.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.