IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.
AuthorsBlom L, Poulsen LK,
JournalJ Immunol
PubMed ID23028054
'The IL-1 family members IL-1ß, IL-18, and IL-33 are potent cytokines in relationship to amplifying the CD4(+) T cell cytokine production. To evaluate their impact on in vitro-differentiated human Th1 and Th2 cultures, such cultures were established from naive T cells, purified from healthy blood donors, and reactivated in the ... More
Multiparameter flow cytometry and bioanalytics for B cell profiling in systemic lupus erythematosus.
AuthorsKaminski DA, Wei C, Rosenberg AF, Lee FE, Sanz I
JournalMethods Mol Biol
PubMed ID22933067
B lymphocyte involvement in systemic lupus erythematosus has been recognized for several decades, mainly in the context of autoantibody production. Both mouse and human studies reveal that different types of antibody responses, as well as antibody-independent effector functions can be ascribed to distinct subpopulations (subsets) of circulating B cells. Characterizing ... More
Evaluation of pneumonia virus of mice as a possible human pathogen.
Pneumonia virus of mice (PVM), a relative of human respiratory syncytial virus (RSV), causes respiratory disease in mice. There is serologic evidence suggesting widespread exposure of humans to PVM. To investigate replication in primates, African green monkeys (AGM) and rhesus macaques (n = 4) were inoculated with PVM by the ... More
A novel aldehyde dehydrogenase-3 activator leads to adult salivary stem cell enrichment in vivo.
AuthorsBanh A, Xiao N, Cao H, Chen CH, Kuo P, Krakow T, Bavan B, Khong B, Yao M, Ha C, Kaplan MJ, Sirjani D, Jensen K, Kong CS, Mochly-Rosen D, Koong AC, Le QT,
JournalClin Cancer Res
PubMed ID21998334
To assess aldehyde dehydrogenase (ALDH) expression in adult human and murine submandibular gland (SMG) stem cells and to determine the effect of ALDH3 activation in SMG stem cell enrichment. Adult human and murine SMG stem cells were selected by cell surface markers (CD34 for human and c-Kit for mouse) and ... More
Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality.
AuthorsChan DA, Sutphin PD, Nguyen P, Turcotte S, Lai EW, Banh A, Reynolds GE, Chi JT, Wu J, Solow-Cordero DE, Bonnet M, Flanagan JU, Bouley DM, Graves EE, Denny WA, Hay MP, Giaccia AJ,
JournalSci Transl Med
PubMed ID21813754
Identifying new targeted therapies that kill tumor cells while sparing normal tissue is a major challenge of cancer research. Using a high-throughput chemical synthetic lethal screen, we sought to identify compounds that exploit the loss of the von Hippel-Lindau (VHL) tumor suppressor gene, which occurs in about 80% of renal ... More
Multiparametric analysis of host response to murine cytomegalovirus in MHC class I-disparate mice reveals primacy of Dk-licensed Ly49G2+ NK cells in viral control.
AuthorsPrince J, Lundgren A, Stadnisky MD, Nash WT, Beeber A, Turner SD, Brown MG,
Journal
PubMed ID24068668
MHC class I D(k) and Ly49G2 (G2) inhibitory receptor-expressing NK cells are essential to murine CMV (MCMV) resistance in MA/My mice. Without D(k), G2(+) NK cells in C57L mice fail to protect against MCMV infection. As a cognate ligand of G2, D(k) licenses G2(+) NK cells for effector activity. These ... More
Self MHC class I-licensed NK cells enhance adaptive CD8 T-cell viral immunity.
AuthorsStadnisky MD, Xie X, Coats ER, Bullock TN, Brown MG
JournalBlood
PubMed ID21436069
MHC class I (MHC I) is essential to NK- and T-cell effector and surveillance functions. However, it is unknown whether MHC I polymorphism influences adaptive immunity through NK cells. Previously, we found that MHC I D(k), a cognate ligand for the Ly49G2 inhibitory receptor, was essential to NK control of ... More
Parallel detection of antigen-specific T cell responses by combinatorial encoding of MHC multimers.
AuthorsAndersen RS, Kvistborg P, Frøsig TM, Pedersen NW, Lyngaa R, Bakker AH, Shu CJ, Straten Pt, Schumacher TN, Hadrup SR
JournalNat Protoc
PubMed ID22498709
Fluorescently labeled multimeric complexes of peptide-MHC, the molecular entities recognized by the T cell receptor, have become essential reagents for detection of antigen-specific CD8(+) T cells by flow cytometry. Here we present a method for high-throughput parallel detection of antigen-specific T cells by combinatorial encoding of MHC multimers. Peptide-MHC complexes ... More
Immunophenotype and cytokine profiles of rhesus monkey CD56bright and CD56dim decidual natural killer cells.
AuthorsDambaeva SV, Durning M, Rozner AE, Golos TG
JournalBiol Reprod
PubMed ID21900681
The primate endometrium is characterized in pregnancy by a tissue-specific population of CD56(bright) natural killer (NK) cells. These cells are observed in human, rhesus, and other nonhuman primate decidua. However, other subsets of NK cells are present in the decidua and may play distinct roles in pregnancy. The purpose of ... More