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View additional product information for Collagen I, bovine - FAQs (A1064401)
11 product FAQs found
The polystyrene that culture flasks are made of tends to have a negative charge. Many cell types will still attach because the plasticware is treated in a way to make them hydrophilic. However cell types that do not attach well to the plastic, or are grown in medium with little or no serum protein, will attach better if the plastic is coated with a protein/polymer which has an overall positive charge.
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An alternative for PureCol, Type I Collagen Solution, 3 mg/ml (Bovine) (Cat. No. 5005) would be Collagen I, bovine (Cat. No. A1064401), which can be found here:
Collagen I, bovine (Cat. No. A1064401)
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Unfortunately, we do not have an animal-origin-free alternative for the Coating Matrix Kit Protein (Cat. No. R011K).However, we offer Rat or Bovine collagen that can be used as coating matrices. Please see the product pages linked below:
Collagen I, bovine (Cat. No. A1064401)
Collagen I, rat tail (Cat. No. A1048301)
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Follow the gelling procedure below to change Collagen I, bovine (Cat. No. A1064401) from liquid to gel.
Gelling procedures (perform under sterile conditions):
1. Place the following on ice:
Collagen (5 mg/mL)
Phosphate buffer saline (10X) or Medium 199 (10X)
Sterile distilled water
Sterile 1N NaOH
2. Determine the concentration and final volume of collagen needed for experimentation. For optimal gel formation, a concentration of 3 or 4 mg/mL is recommended.
3. Determine the amount of reagents needed to achieve the desired collagen concentration in 1X PBS or Medium 199 with normal osmolality and neutral pH.
- V = total volume of collagen gel desired
- Volume of collagen needed (V1) = (Final conc. of collagen) x ( total volume (V))/Initial concentration of collagen
- Volume of 10X PBS needed (V2) = Total volume (V)/10
- Volume of 1N NaOH needed (V3) = (total volume of collagen needed (V1)) x 0.025
- Volume of dH2O needed (V4) = Total volume – (calculated volumes from steps V1 + V2 + V3)
4. Mix dH2O, 1N NaOH, and 10X PBS in a sterile tube.
5. Rinse the gel with 1X PBS or cell culture medium before seeding cells.
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An alternative for Collagen I, rat tail (Cat. No A1048301) is Collagen I, bovine (Cat. No A1064401). While the source of Collagen I is different, they can both be prepared as a clear gel providing a 3D matrix or surface coating on tissue culture plates as a substrate for culturing primary cells such as keratinocytes and hepatocytes.
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Collagen I, bovine (Cat. No. A1064401) is shipped in 20 mM acetic acid.
Collagen I is provided as a liquid, suspended in 20 mM acetic acid solution. If difficulties are encountered with the preparation, the collagen can be diluted in 1:2, first in 20 mM acetic acid, pH 3.5 before further diluting according to the protocol being used.
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Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.
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The following cell types are commonly cultured in collagen I:
- Endothelial cells (including HUVEC)
- Fibroblasts
- Hepatocytes
- Chondrocytes
- Osteoclasts
- Osteoblasts
- Keratinocytes
- Corneal epithelium
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The key applications include the following:
- Collagen I induces microvascular endothelial cells to adopt a spindle-shaped morphology, in vitro, and to align into solid cord-like assemblies. Vascular endothelial cells can also form vessel-like, tubular structures when cultured on collagen I. Collagen I can be used for in vitro angiogenesis assays.
- Breast cancer stem cells can undergo differentiation when cultured on collagen I.
- Collagen I has been used for the culture of primary colon carcinoma cell lines; mouse liver progenitor cells have been cultured in 3D collagen I and rat pancreatic islets.
- Collagen I can act as a barrier in cell invasion assays, and has been used to study cell adhesion.
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Gibco Geltrex Matrix and collagen (rat tail and bovine) can be used as either a coating solution or a 3D gel matrix. CELLstart Substrate was developed to be used as a xeno-free coating matrix for only ESC applications (as a substitute for Gibco Geltrex Matrix or Matrigel Matrix, which are of animal origin). AlgiMatrix Matrix is a 3D scaffold-type matrix that does not support cell attachment but does provide a good environment for growing spheroids that can be easily harvested for downstream applications.
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