StemPro™ Accutase™ Cell Dissociation Reagent - FAQs

No. However, although StemPro Accutase is gentle on cells, the optimal time for dissociation should be empirically determined for your specific cell type and application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

No. StemPro Accutase is supplied as a convenient, ready to use reagent. No dilution is required prior to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

No. StemPro Accutase is gentle enough that only dilution of the reagent with DPBS or media is required to stop the dissociation activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend storing StemPro Accutase Cell Dissociation Reagent at -5 to -20 degrees C, protected from light. We do not recommend subjecting it to more than 3-4 freeze-thaws. When stored at 2 to 8 degrees C, the reagent is stable for up to 2 years.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. If StemPro Accutase is exposed to 37 degrees C just until complete thawing is achieved, it still can be used. However, this could result in decreased enzyme activity and it would take more time to get full dissociation. If you observe this, consider starting with a new bottle. Please note that if a bottle of StemPro Accutase is kept at 37 degrees C for more than one hour, it will lose its activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes, as long as the StemPro Accutase is still cool to the touch, it should be okay to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. We recommend thawing it at room temperature or overnight at 4 degrees C to get complete thawing. Otherwise, place in the freezer and refreeze.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. During the freezing procedure or shipping, uneven color can be observed. However, this will not compromise the activity or performance of the enzyme. Just make sure to mix the solution by inverting the tube or bottle to get an even color before use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. StemPro Accutase has been tested to dissociate spheres of neural progenitors (neurospheres).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend using StemPro Accutase wherever gentle and efficient dissociation is needed. We have also validated StemPro Accutase for use with hESC grown in StemPro hESC SFM and for NSC grown in StemPro NSC SFM. All the tests resulted in great recovery of cells without losing their properties and while maintaining good viability. In addition, the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/5029_A1110501_Accutase.pdf) lists examples of cell lines where the application has been validated and published.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend using EDTA for passaging PSCs. For seeding cells for definitive endoderm induction, we recommend using StemPro Accutase Cell Dissociation Reagent for small clumps and Gibco TrypLE Enzyme for singularized cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.

Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.