StemPro® Accutase® Cell Dissociation Reagent

Catalog number: A1110501

Gibco™  Related applications: Hepatocytes & Hepatic Models | Mammalian Cell Culture | Pharma & Biopharma | Primary Cell Culture | Stem Cell Culture

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Description

StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase® application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells. A complete list of published references is available on our website. Accutase® is a trademark of ICT, Inc.
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.

Specifications

Classification: Serum-Free
Reagent Type: Accutase®
Cell Type: Stem Cells
Form: Frozen
Inorganic Salts: Sodium Phosphate
Product Size: 100 mL
Purity or Quality Grade: Research Grade
Shipping Condition: Dry Ice

Contents & storage

Accutase® is supplied as a ready-to-use cell dissociation reagent. Store Accutase® at -20°C upon receipt. After thawing, Accutase® may be stored for up to 2 years at 4°C. Multiple freeze thaws are not recommended. DO NOT STORE AT ROOM TEMP

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