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Invitrogen
GFP Monoclonal Antibody (11E5)
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FIGURE: 1 / 33
GFP Antibody (A-11121) in IHC (P), ICC/IF
eLife 2019 -
Figure 3. The endocytic zone is mislocalized in Drp1 exonA -KO neurons. ( A and B ) Hippocampal neurons were cultured and transfected with plasmids expressing Psd-95.FingR-GFP and mCherry-CLC. Two days after transfection, neurons were subjected to chemical LTD stimulation (NMDA/Gly), fixed and analyzed by laser scanning confocal microscopy. Bar, 5 um. The number of Psd-95.FingR-GFP signals that are not asso... View More
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.jpg?time=20240507?time=20240507 "GFP Antibody (A-11121) in WB")
Product Details
A-11121
Applications
Tested Dilution
Publications
Product Specifications
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
11E5
Immunogen
The GFP was isolated directly from the jellyfish Aequorea victoria.
Conjugate
Form
Lyophilized
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Product Specific Information
The anti-GFP antibody is suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis. Anti-GFP mouse monoclonal antibody 11E5 is optimized for western analysis, allowing colorimetric detection of as little as 10 ng of GFP or GFP-fusion proteins, or chemiluminescent detection of picogram quantities.
These antibodies have been purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA before lyophilization. At the time of preparation, the products are certified to be free of unconjugated dyes. They are tested in a cytological experiment to ensure low nonspecific staining.
To prepare 0.2 mg/mL stock solutions, reconstitute the lyophilized antibodies in 0.5 mL of phosphate-buffered saline (PBS), pH 7.4. You may store these solutions for up to 3 months at 4°C with the addition of 2 mM sodium azide.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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