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Invitrogen
Bovine Serum Albumin Polyclonal Antibody
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FIGURE: 1 / 15
Bovine Serum Albumin Antibody (A11133) in WB
Western blot analysis was performed by loading 500 ng (Lane 1), 250 ng (Lane 2), 125 ng (Lane 3) and 60 ng (Lane 4) of BSA. The blot was probed with Anti- Bovine Serum Albumin Rabbit Polyclonal Antibody (Product # A11133, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A11133
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Published species
Baboon,
Bovine,
Human,
Mouse,
Non-human primate,
Rabbit
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Bovine Serum Albumin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Amount
1 mg
Purification
IgG fraction
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Anti-bovine serum albumin (anti-BSA) antibody is a highly purified rabbit IgG fraction that can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and little or no crossreactivity to other serum proteins. Cat. No. A11133 can be used in enzyme and fluorescence immunoassays and blots, cell or tissue immunostaining, and as a precipitating antibody or as an immuno-adsorbant when attached to solid supports. For detection of this rabbit antibody, we offer a variety of second-step reagents labeled with biotin or with a wide range of fluorophores.
Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:100 to 1:1,000 to determine the optimal conditions for your experiments.
Target Information
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bovine albumin; BSA; MGC179277; serum albumin
UniProt ID: (Bovine) P02769
Entrez Gene ID: (Bovine) 280717
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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