The Amplex® Red Glutamic Acid/Glutamate Oxidase Assay Kit provides a sensitive and simple method for detecting glutamic acid/glutamate oxidase activity using a fluorescence microplate reader or fluorometer.
• Detect concentrations as low as 10 nM L-glutamic acid or 40 µU/mL of purified L-glutamate oxidase • Format allows for multiple time point measurements • Designed for minimal autofluorescence interference
The Amplex® Red Glutamic Acid/Glutamate Oxidase Assay Kit provides an ultrasensitive method for detecting glutamic acid or for continuously monitoring glutamate oxidase activity using a fluorescence microplate reader or fluorometer. In the assay, L-glutamic acid is oxidized by glutamate oxidase to produce α-ketoglutarate, NH3, and hydrogen peroxide. L-alanine and L-glutamate–pyruvate transaminase are included in the reaction to regenerate L-glutamic acid by transamination of α-ketoglutarate, resulting in multiple cycles of the initial reaction and a significant amplification of the hydrogen peroxide produced. The hydrogen peroxide reacts with 10-acetyl-3,7-dihydroxyphenoxazine (Amplex® Red reagent) in a 1:1 stoichiometric ratio in a reaction catalyzed by horseradish peroxidase (HRP) to generate the highly fluorescent product resorufin.
Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.
Use Amplex® Red Assays for a Broad Range of Investigations A wide variety of validated Amplex® Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex® UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex® Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.