Glutamate excitotoxicity is involved in cell death caused by tributyltin in cultured rat cortical neurons.
AuthorsNakatsu Y, Kotake Y, Komasaka K, Hakozaki H, Taguchi R, Kume T, Akaike A, Ohta S,
JournalToxicol Sci
PubMed ID16207939
'Tributyltin, an endocrine-disrupting chemical, has been used as a heat stabilizer, agricultural pesticide, and component of antifouling paints. In this study, the neurotoxicity of tributyltin was investigated in cultured rat cortical neurons. Tributyltin caused marked time- and dose-dependent increases in the number of trypan blue-stained cells. Measurement of extracellular glutamate ... More
Iron alters glutamate secretion by regulating cytosolic aconitase activity.
AuthorsMcGahan MC, Harned J, Mukunnemkeril M, Goralska M, Fleisher L, Ferrell JB
JournalAm J Physiol Cell Physiol
PubMed ID15613494
'Glutamate has many important physiological functions, including its role as a neurotransmitter in the retina and the central nervous system. We have made the novel observations that retinal pigment epithelial cells underlying and intimately interacting with the retina secrete glutamate and that this secretion is significantly affected by iron. In ... More
Bispecific small molecule-antibody conjugate targeting prostate cancer.
'Bispecific antibodies, which simultaneously target CD3 on T cells and tumor-associated antigens to recruit cytotoxic T cells to cancer cells, are a promising new approach to the treatment of hormone-refractory prostate cancer. Here we report a site-specific, semisynthetic method for the production of bispecific antibody-like therapeutics in which a derivative ... More
Single cell glutamate analysis in Aplysia sensory neurons.
AuthorsDrake TJ, Jezzini S, Lovell P, Moroz LL, Tan W
JournalJ Neurosci Methods
PubMed ID15848241
'Glutamate is the major excitatory neurotransmitter in the central nervous system. However, techniques and assays available for the determination and detection of glutamate are limited. Here we have applied an effective glutamate assay toward the high-throughput analysis of single neurons. Initial physiological studies and recent immunohistochemical data strongly suggested that ... More
P2X agonist BzATP interferes with amplex-red-coupled fluorescence assays.
AuthorsLeón D, Marín-García P, Sánchez-Nogueiro J, de la O FO, García-Carmona F, Miras-Portugal MT,
JournalAnal Biochem
PubMed ID17562321
'This paper does not have an abstract.'
Astrocyte-induced cortical vasodilation is mediated by D-serine and endothelial nitric oxide synthase.
AuthorsStobart JL, Lu L, Anderson HD, Mori H, Anderson CM,
JournalProc Natl Acad Sci U S A
PubMed ID23386721
'Astrocytes play a critical role in neurovascular coupling by providing a physical linkage from synapses to arterioles and releasing vaso-active gliotransmitters. We identified a gliotransmitter pathway by which astrocytes influence arteriole lumen diameter. Astrocytes synthesize and release NMDA receptor coagonist, D-serine, in response to neurotransmitter input. Mouse cortical slice astrocyte ... More
Production of reactive oxygen species by mitochondria: central role of complex III.
AuthorsChen Q, Vazquez EJ, Moghaddas S, Hoppel CL, Lesnefsky EJ
JournalJ Biol Chem
PubMed ID12840017
'The mitochondrial respiratory chain is a major source of reactive oxygen species (ROS) under pathological conditions including myocardial ischemia and reperfusion. Limitation of electron transport by the inhibitor rotenone immediately before ischemia decreases the production of ROS in cardiac myocytes and reduces damage to mitochondria. We asked if ROS generation ... More
The functional role of the second NPXY motif of the LRP1 beta-chain in tissue-type plasminogen activator-mediated activation of N-methyl-D-aspartate receptors.
AuthorsMartin AM, Kuhlmann C, Trossbach S, Jaeger S, Waldron E, Roebroek A, Luhmann HJ, Laatsch A, Weggen S, Lessmann V, Pietrzik CU,
JournalJ Biol Chem
PubMed ID18321860
The low density lipoprotein receptor-related protein 1 (LRP1) emerges to play fundamental roles in cellular signaling pathways in the brain. One of its prominent ligands is the serine proteinase tissue-type plasminogen activator (tPA), which has been shown to act as a key activator of neuronal mitogen-activated protein kinase pathways via ... More
Involvement of sphingosine-1-phosphate in glutamate secretion in hippocampal neurons.
Neuronal activity greatly influences the formation and stabilization of synapses. Although receptors for sphingosine-1-phosphate (S1P), a lipid mediator regulating diverse cellular processes, are abundant in the central nervous system, neuron-specific functions of S1P remain largely undefined. Here, we report two novel actions of S1P using primary hippocampal neurons as a ... More
Aminoglutethimide prevents excitotoxic and ischemic injuries in cortical neurons.
AuthorsShirakawa H, Katsuki H, Kume T, Kaneko S, Akaike A,
JournalBr J Pharmacol
PubMed ID16474421
Aminoglutethimide is a clinically available drug that suppresses steroid biosynthesis by inhibiting enzymes such as cytochrome P450scc and aromatase. Because several members of neurosteroids regulate glutamate receptors, we investigated the effect of aminoglutethimide on cell death induced by overactivation of glutamate receptors in CNS neurons. Long-term pretreatment of organotypic cerebrocortical ... More
BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: roles in synaptic modulation.
AuthorsSuzuki S, Numakawa T, Shimazu K, Koshimizu H, Hara T, Hatanaka H, Mei L, Lu B, Kojima M
JournalJ Cell Biol
PubMed ID15596541
Brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity but the underlying signaling mechanisms remain unknown. Here, we show that BDNF rapidly recruits full-length TrkB (TrkB-FL) receptor into cholesterol-rich lipid rafts from nonraft regions of neuronal plasma membranes. Translocation of TrkB-FL was blocked by Trk inhibitors, suggesting a ... More
Isolation of amniotic stem cell lines with potential for therapy.
AuthorsDe Coppi P, Bartsch G, Siddiqui MM, Xu T, Santos CC, Perin L, Mostoslavsky G, Serre AC, Snyder EY, Yoo JJ, Furth ME, Soker S, Atala A
JournalNat Biotechnol
PubMed ID17206138
Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines ... More
Enzyme-linked sensitive fluorometric imaging of glutamate release from cerebral neurons of chick embryos.
AuthorsAkagi Y, Hashigasako A, Degenaar P, Iwabuchi S, Hasan Q, Morita Y, Tamiya E
JournalJ Biochem (Tokyo)
PubMed ID14561720
This paper describes a method for imaging the endogenous release of glutamate from cerebral neurons. This method is based on the reactions of glutamate oxidase and peroxidase, and on the detection of hydrogen peroxide by a fluorescent substrate of peroxidase. Glutamate has been sensitively measured in vitro in the range ... More