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Hibernate™-E Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A1247601

Gibco™  Related applications: Stem Cell Culture

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Hibernate® is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate®-A is the nutrient medium for adult and postnatal cells or tissue in ambient carbon dioxide levels; Hibernate®-E is the nutrient medium for embryonic cells or tissue in ambient carbon dioxide levels. The first application allows for longer period (up to 48 hours) maintenance of neuron viability in ambient CO2. This gives scientists greater convenience and control running bench-top live cell experiments, such as microscopy studies, flow cytometry, and other physiology studies. The second application of Hibernate® is for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

• Optimized media systems for the viable storage of neural tissue and slices for up to 1 month at 2 - 8 °C - convenient systems that allow scientists to preserve tissue⁄slices before processing

• Maintenance of neuronal cells viability and functions at ambient CO2 level for up to 48 hours - makes conducting live-cell experiments possible without an expensive CO2 chamber adapted to instrument

• Manufactured under cGMP.

GIBCO® Hibernate®-A and Hibernate®-E are CO2-independent nutrient media for the maintenance of neural cells and preservation of tissue. It is supplied as 1X medium and mixing of Hibernate® with GIBCO® B-27® Supplement (50X) yields a complete medium.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.


1. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month.Brewer GJ, Price P J.Neuroreport. 1996 Jun 17;7(9):1509-12.
2. Serum-free culture of rat post-natal and fetal brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Dev Brain Res. 2000 Apr 14;120(2):199-210.
3. Method for serum-free culture of late fetal and early postnatal rat brainstem neurons.Kivell BM, McDonald FJ, Miller JH. Brain Res Brain Res Protoc. 2001 Feb;6(3):91-9.
4. Human primary brain tumor cell growth inhibition in serum-free medium optimized for neuron survival.Brewer GJ, LeRoux PD. Brain Res. 2007 Jul 9;1157:156-66.
5. Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice.Vieira M, Christensen BL, Wheeler BC, Feng AS, Kollmar R. Hear Res. 2007 Aug;230(1-2):17-23.
6. Dopamine D1-like receptor activation induces brain-derived neurotrophic factor protein expression.Williams SN, Undieh AS. Neuroreport. 2009 Apr 22;20(6):606-10.
7. Neonatal desensitization allows long-term survival of neural xenotransplants without immunosuppression.Kelly CM, Precious SV, Scherf C, Penketh R, Amso NN, Battersby A, Allen ND, Dunnett SB, Rosser AE. Nature Methods. 2009 Apr;6(4):271-3.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells, Neuronal Cells (Post-Natal), Neuronal Cells (Pre-Natal), Neuronal Tumor Cells, Primary Cells
Amino Acids: No Glutamic Acid
Classification: Animal Origin-Free, Chemically Defined
Concentrated: 1 X
Culture Environment: Non-CO2
Endotoxin Level: < 1 EU/ml
Form: Liquid
Glucose: High Glucose
Glutamine: No Glutamine
Included Antibiotics: No antibiotics
Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 500 mL
Purity or Quality Grade: Cell Culture Grade, Molecular Biology Grade
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Sterility: Sterile
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

1X liquid store at 2-8°C in the dark.


Manuals & protocols