Invitrogen™

Biocytin Alexa Fluor™ 488 (Alexa Fluor™ 488 Biocytin, Disodium Salt)

Catalog number:  A12924

Related applications:

Cell Tracing & Tracking

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Description

The cell-impermeant, fixable, polar tracer Alexa Fluor® 488 biocytin combines the green-fluorescent Alexa Fluor® 488 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF technologies, respectively.

Specifications

Label Type: Alexa Fluor Dyes
Product Line: Alexa Fluor™
Reagent Type: Biocytins
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.