Deconvolving single-molecule intensity distributions for quantitative microscopy measurements.
AuthorsMutch SA, Fujimoto BS, Kuyper CL, Kuo JS, Bajjalieh SM, Chiu DT,
JournalBiophys J
PubMed ID17259276
'In fluorescence microscopy, images often contain puncta in which the fluorescent molecules are spatially clustered. This article describes a method that uses single-molecule intensity distributions to deconvolve the number of fluorophores present in fluorescent puncta as a way to "count" protein number. This method requires a determination of the correct ... More
Hebbian LTP in feed-forward inhibitory interneurons and the temporal fidelity of input discrimination.
AuthorsLamsa K, Heeroma JH, Kullmann DM
JournalNat Neurosci
PubMed ID15937481
'Cortical information processing requires a delicate balance of excitatory and inhibitory signaling. How is this balance preserved during hippocampal memory encoding, which involves NMDA receptor-dependent long term potentiation (LTP)? This form of LTP occurs at synapses between pyramidal neurons but has not been detected in feed-forward inhibitory interneurons. We show ... More
Evolutionary origins for social vocalization in a vertebrate hindbrain-spinal compartment.
AuthorsBass AH, Gilland EH, Baker R,
JournalScience
PubMed ID18635807
'The macroevolutionary events leading to neural innovations for social communication, such as vocalization, are essentially unexplored. Many fish vocalize during female courtship and territorial defense, as do amphibians, birds, and mammals. Here, we map the neural circuitry for vocalization in larval fish and show that the vocal network develops in ... More
Electrophysiological properties of morphologically-identified medial vestibular nucleus neurons projecting to the abducens nucleus in the chick embryo.
AuthorsGottesman-Davis A, Shao M, Hirsch JC, Peusner KD,
JournalNeuroscience
PubMed ID20971163
'Neurons in the medial vestibular nucleus (MVN) show a wide range of axonal projection pathways, intrinsic firing properties, and responses to head movements. To determine whether MVN neurons participating in the vestibulocular reflexes (VOR) have distinctive electrophysiological properties related to their output pathways, a new preparation was devised using transverse ... More
The styryl dye FM1-43 suppresses odorant responses in a subset of olfactory neurons by blocking cyclic nucleotide-gated (CNG) channels.
AuthorsBreunig E, Kludt E, Czesnik D, Schild D,
JournalJ Biol Chem
PubMed ID21646359
'Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with ... More
Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo.
AuthorsValadon P, Darsow B, Buss TN, Czarny M, Griffin NM, Nguyen HN, Oh P, Borgstrom P, Chrastina A, Schnitzer JE,
JournalJ Biol Chem
PubMed ID19850928
Molecular medicine can benefit greatly from antibodies that deliver therapeutic and imaging agents to select organs and diseased tissues. Yet the development of complex and defined composite nanostructures remains a challenge that requires both designed stoichiometric assembly and superior in vivo testing ability. Here, we generate nanostructures called nanostreptabodies by ... More
Octavolateral projections and organization in the medulla of a teleost fish, the sleeper goby (Dormitator latifrons).
AuthorsTomchik SM, Lu Z,
JournalJ Comp Neurol
PubMed ID15558734
This study is the first to employ simultaneous labeling with different colored fluorescent dyes and confocal microscopy to investigate the central projections of the octavolateral nerves in any fish. Three-dimensional reconstructions of the hindbrain octavolateral nuclei were made and overlap of octavolateral projections was assessed in a teleost, the sleeper ... More
Multiplexed protein analysis using encoded antibody-conjugated microbeads.
AuthorsTheilacker N, Roller EE, Barbee KD, Franzreb M, Huang X,
JournalJ R Soc Interface
PubMed ID21247950
We describe a method for multiplexed analysis of proteins using fluorescently encoded microbeads. The sensitivity of our method is comparable to the sensitivity obtained by enzyme-linked immunosorbent assay while only 5 µl sample volumes are needed. Streptavidin-coated, 1 µm beads are encoded with a combination of fluorophores at different intensity ... More
Light-directed electrical stimulation of neurons cultured on silicon wafers.
AuthorsStarovoytov A, Choi J, Seung HS
JournalJ Neurophysiol
PubMed ID15385589
Dissociated neurons cultured in vitro can serve as a model system for studying the dynamics of neural networks. Such studies depend on techniques for stimulating patterns of neural activity. We show a technique for extracellular stimulation of dissociated neurons cultured on silicon wafers. When the silicon surface is reverse biased, ... More