Gbetagamma activation of Src induces caveolae-mediated endocytosis in endothelial cells.
AuthorsShajahan AN, Tiruppathi C, Smrcka AV, Malik AB, Minshall RD,
JournalJ Biol Chem
PubMed ID15345719
'Caveolae-mediated endocytosis in endothelial cells is stimulated by the binding of albumin to gp60, a specific albumin-binding protein localized in caveolae. The activation of gp60 induces its cell surface clustering and association with caveolin-1, the caveolar-scaffolding protein. This interaction leads to G(i)-induced Src kinase activation, which in turn signals dynamin-2-mediated ... More
Albumin mediates the transcytosis of myeloperoxidase by means of caveolae in endothelial cells.
AuthorsTiruppathi C, Naqvi T, Wu Y, Vogel SM, Minshall RD, Malik AB,
JournalProc Natl Acad Sci U S A
PubMed ID15136724
'Myeloperoxidase (MPO), the phagocyte hemoprotein involved in neutrophil host defense and consuming nitric oxide (*NO), induces the nitration of extracellular matrix proteins and tissue remodeling subsequent to its transcytosis across the endothelial barrier. We addressed the role of an interaction of MPO with albumin as a requirement for MPO transport ... More
Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF.
AuthorsMacounová K, Cabrera CR, Yager P
JournalAnal Chem
PubMed ID11321320
'The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 ... More
Real-time differential labeling of blood, interstitium, and lymphatic and single-field analysis of vasculature dynamics in vivo.
'Lymph nodes are highly organized structures specialized for efficient regulation of adaptive immunity. The blood and lymphatic systems within a lymph node play essential roles by providing functionally distinct environments for lymphocyte entry and egress, respectively. Direct imaging and measurement of vascular microenvironments by intravital multiphoton microscopy provide anatomical and ... More
Initiation and termination of NF-kappaB signaling by the intracellular protozoan parasite Toxoplasma gondii.
AuthorsShapira S, Harb OS, Margarit J, Matrajt M, Han J, Hoffmann A, Freedman B, May MJ, Roos DS, Hunter CA,
JournalJ Cell Sci
PubMed ID16079291
Signaling via the NF-kappaB cascade is critical for innate recognition of microbial products and immunity to infection. As a consequence, this pathway represents a strong selective pressure on infectious agents and many parasitic, bacterial and viral pathogens have evolved ways to subvert NF-kappaB signaling to promote their survival. Although the ... More
A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells.
AuthorsLarsen EC, Ueyama T, Brannock PM, Shirai Y, Saito N, Larsson C, Loegering D, Weber PB, Lennartz MR
JournalJ Cell Biol
PubMed ID12499353
Protein kinase C (PKC) plays a prominent role in immune signaling, and the paradigms for isoform selective signaling are beginning to be elucidated. Real-time microscopy was combined with molecular and biochemical approaches to demonstrate a role for PKC- epsilon in Fc gamma receptor (Fc gammaR)-dependent phagocytosis. RAW 264.7 macrophages were ... More
Characterizing the cellular architecture of dynamically remodeling vascular tissue using 3-D image analysis and virtual reconstruction.
Authors
JournalMol Biol Cell
PubMed ID32614644
Vascular regeneration in a basal chordate is due to the presence of immobile, bi-functional cells.
Authors
JournalPLoS One
PubMed ID24736432
Th17 lymphocytes drive vascular and neuronal deficits in a mouse model of postinfectious autoimmune encephalitis.
In vivo manipulation of the extracellular matrix induces vascular regression in a basal chordate.
Authors
JournalMol Biol Cell
PubMed ID28615322
Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds.
Authors
JournalActa Biomater
PubMed ID21601017
EphB1 interaction with caveolin-1 in endothelial cells modulates caveolae biogenesis.
Authors
JournalMol Biol Cell
PubMed ID32238105
The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels.
Authors
JournalNeuron
PubMed ID29154126
Evaluation of Glomerular Hemodynamic Function by Empagliflozin in Diabetic Mice Using In Vivo Imaging.
Authors
JournalCirculation
PubMed ID30773020
Loss of Christianson Syndrome Na+/H+ Exchanger 6 (NHE6) Causes Abnormal Endosome Maturation and Trafficking Underlying Lysosome Dysfunction in Neurons.