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Freedom® CHO-S® Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: A1369601

Gibco™  Related applications: Antibody & Cell Culture Production | Cell Line Development for Protein Production | Protein Expression

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Gibco® Freedom® CHO-S® Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. The Gibco® Freedom® CHO-S® Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco® Freedom® CHO-S® Kit also delivers high titers, and offers simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3g/L
• Complete workflow that can take your gene of interest from transfection to lead clone typically in 5 months
• Convenient packaging, for simplified ordering and storage

Commercial Licensing that is Simple, Flexible and Without Royalties
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at

Industry Standard Production Levels
Current bioprocess industry standards to achieve manufacturing efficiencies for IgG type molecule production with stable CHO cell lines in bioreactors is 2-3g/L or better. We have demonstrated that the Gibco® Freedom® CHO-S® Kit can consistently generate IgG producing scalable clones (stable out to 60 population doublings), that meet this standard of productivity (Fig 2).

Complete, Fast, and Efficient Workflow
The Gibco® Freedom® CHO-S® Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in 5 months, with reduced hands on time, and reduced Fulltime Equivalent (FTE) requirements (Fig 3). The entire workflow is performed in our CD FortiCHO™ medium, a chemically defined, animal origin free (AOF) medium. The kit also offers the following advantages:

• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into CHO-S® Cells (cGMP-banked)
• Included flash drive with loaded manual

New Convenient Packaging
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® CHO-S® Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.

Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV⁄EF1 Hybrid Promoter, EF2⁄CMV Hybrid Promoter
Quantity: 1 kit
Cell Line: CHO-S Cells, cGMP Banked
Expression: Mammalian
Key Function: Protein Production, Stable Cell Line Development
Product Size: 1 kit
Delivery Method: Transfection
Selection Agent (Eukaryotic): MTX (Methotrexate), Puromycin
Selection Marker (Eukaryotic): PurR, DHFR
Green Features: Fewer resources used, Less waste

Contents & storage

CD FortiCHO™ Medium: 2°C to 8°C, protect from light
OptiPRO™ SFM: 2°C to 8°C, protect from light
FreeStyle™ MAX Reagent: 2°C to 8°C, do not freeze

CHO–S™ Cells (cGMP–banked): liquid nitrogen
One Shot® TOP10 Chemically Competent E. coli: –80°C
Freedom® pCHO 1.0 Expression Vector: –5°C to –20°C


Manuals & protocols

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