FreeStyle™ F17 Expression Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A1383502

Related applications:

Antibody & Cell Culture Production

| Mammalian Cell Culture

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Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

• Little or no required adaptation
• Supports suspension growth and transfection of HEK293 cells
• Chemically defined, protein-free, serum-free formulation
• Compatible with multiple transient production systems
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle F17 medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Growth and transfection of HEK293 cells
FreeStyle F17 Expression Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL. Cells cultured in FreeStyle F17 medium can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent.

Scalability in spinner flasks and bioreactors
Production using FreeStyle F17 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement FreeStyle F17 medium with additional Pluronic F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
FreeStyle F17 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Design your own medium specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco cGMP Media Custom Services you can put the manufacturing power and years of technical experience to work for you.


Concentration: 1 X
Sterility: Sterile
Cell Line: HEK293
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Concentration Ratio: 1 :1
Culture Environment: CO2
Endotoxin Level: < 1 EU/ml
Purity or Quality Grade: Qualified for Further Manufacturing
Serum Level: Serum-Free
Supplements: 4–8 mM L-glutamine or GlutaMAX™
Volume (Metric): 1000 ml
Product Line: Freestyle™
Form: Liquid
Glutamine: No Glutamine
Green Features: Sustainable packaging
Quantity: 6 bottle(s)
Format: Bottle(s)
Culture Type: Suspension Cell Culture
For Use With (Application): Protein expression, viral vector production

Contents & storage

Contains: 6 × 1 L bottles
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture