'Three-dimensional (3D) hydrogel structures are finding use in fundamental studies of self-assembly, rheology, and 3D cell culture. Most techniques for 3D hydrogel formation are ''single pot'', in which gels are not addressable after formation. For many applications, it would be useful to be able to form arrays of gels bearing ... More
Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells.
AuthorsTaniguchi K, Shao Y, Townshend RF, Tsai YH, DeLong CJ, Lopez SA, Gayen S, Freddo AM, Chue DJ, Thomas DJ, Spence JR, Margolis B, Kalantry S, Fu J, O'Shea KS, Gumucio DL
Journal
PubMed ID26626176
'We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs form two-cell cysts with a shared apical domain within 20 hr of plating; these cysts collapse to form monolayers after 5 days. Expression of pluripotency markers is maintained throughout this time. In two-cell cysts, ... More
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
AuthorsSatelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman MJ, Kopetz S, Ellis LM, Meng QH, Li S
Journal
PubMed ID25516888
This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers. ... More
AuthorsTheinHan W, Liu J, Tang M, Chen W, Cheng L, Xu HH
Journal
PubMed ID24839581
Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs ... More
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.
AuthorsSong L, Tsai AC, Yuan X, Bejoy J, Sart S, Ma T, Li Y
JournalTissue Eng Part A
PubMed ID29160172
'Organoids, the condensed three-dimensional (3D) tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types, such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs)-derived neural progenitors aid neural organ development, ... More
Adult Tissue Extracellular Matrix Determines Tissue Specification of Human iPSC-Derived Embryonic Stage Mesodermal Precursor Cells.
AuthorsUllah I, Busch JF, Rabien A, Ergün B, Stamm C, Knosalla C, Hippenstiel S, Reinke P, Kurtz A
JournalAdv Sci (Weinh)
PubMed ID32154066
'The selection of pluripotent stem cell (PSC)-derived cells for tissue modeling and cell therapy will be influenced by their response to the tissue environment, including the extracellular matrix (ECM). Whether and how instructive memory is imprinted in adult ECM and able to impact on the tissue specific determination of human ... More
3D bioprinted mammary organoids and tumoroids in human mammary derived ECM hydrogels.
AuthorsMollica PA, Booth-Creech EN, Reid JA, Zamponi M, Sullivan SM, Palmer XL, Sachs PC, Bruno RD
JournalActa Biomater
PubMed ID31233891
'The extracellular matrix (ECM) of tissues is an important mediator of cell function. Moreover, understanding cellular dynamics within their specific tissue context is also important for developmental biology, cancer research, and regenerative medicine. However, robust in vitro models that incorporate tissue-specific microenvironments are lacking. Here we describe a novel mammary-specific ... More
Microengineered human amniotic ectoderm tissue array for high-content developmental phenotyping.
AuthorsNasr Esfahani S, Shao Y, Resto Irizarry AM, Li Z, Xue X, Gumucio DL, Fu J
JournalBiomaterials
PubMed ID31207406
'During early post-implantation human embryogenesis, the epiblast (EPI) within the blastocyst polarizes to generate a cyst with a central lumen. Cells at the uterine pole of the EPI cyst then undergo differentiation to form the amniotic ectoderm (AM), a tissue essential for further embryonic development. While the causes of early ... More
Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche.
AuthorsShao Y, Taniguchi K, Gurdziel K, Townshend RF, Xue X, Yong KMA, Sang J, Spence JR, Gumucio DL, Fu J
JournalNat Mater
PubMed ID27941807
Amniogenesis-the development of amnion-is a critical developmental milestone for early human embryogenesis and successful pregnancy. However, human amniogenesis is poorly understood due to limited accessibility to peri-implantation embryos and a lack of in vitro models. Here we report an efficient biomaterial system to generate human amnion-like tissue in vitro through self-organized development ... More
Human retinal endothelial cells and astrocytes cultured on 3-D scaffolds for ocular drug discovery and development.
Topical ocular ketorolac improves the outcomes of severe retinopathy of prematurity and when administered with systemic caffeine, decreases the severity of oxygen-induced retinopathy. We tested the hypothesis that co-cultures of human retinal endothelial cells (HRECs) and human retinal astrocytes (HRAs) on 3-dimensional (3-D) hydrogel scaffolds is a more representative biomimetic ... More
Hydrogels in three-dimensional dendritic cell (MUTZ-3) culture as a scaffold to mimic human immuno competent subcutaneous tissue.
AuthorsGroell F, Kalia YN, Jordan O, Borchard G
JournalInt J Pharm
PubMed ID29698823
The objective of this study was to develop a 3D cell culture model of the human subcutaneous tissue, allowing the prediction of the immunogenicity of subcutaneously injected therapeutic proteins. Several hydrogels were evaluated as scaffolds to mimic the human subcutaneous tissue in vitro. Cytocompatibility of the hydrogels with the human ... More
Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues.
AuthorsSong L, Yuan X, Jones Z, Griffin K, Zhou Y, Ma T, Li Y
JournalSci Rep
PubMed ID30979929
Human cerebral organoids derived from induced pluripotent stem cells (iPSCs) provide novel tools for recapitulating the cytoarchitecture of human brain and for studying biological mechanisms of neurological disorders. However, the heterotypic interactions of neurovascular units, composed of neurons, pericytes, astrocytes, and brain microvascular endothelial cells, in brain-like tissues are less ... More
Differentiation and characterization of neurons derived from rat iPSCs.
AuthorsSetien MB, Smith KR, Howard K, Williams K, Suhr ST, Purcell EK
JournalJ Neurosci Methods
PubMed ID32199944
Induced pluripotent stem cells (iPSCs) may be an advantageous source of neuronal cells to repair damage due to neurological disorders or trauma. Additionally, they are promising candidates to develop models to study underlying mechanisms of neurodegenerative diseases. While successful neural differentiation of iPSCs has been reported in mice, protocols detailing ... More
Enhanced structural maturation of human induced pluripotent stem cell-derived cardiomyocytes under a controlled microenvironment in a microfluidic system.
AuthorsKolanowski TJ, Busek M, Schubert M, Dmitrieva A, Binnewerg B, Pöche J, Fisher K, Schmieder F, Grünzner S, Hansen S, Richter A, El-Armouche A, Sonntag F, Guan K
JournalActa Biomater
PubMed ID31778832
The lack of a fully developed human cardiac model in vitro hampers the progress of many biomedical research fields including pharmacology, developmental biology, and disease modeling. Currently, available methods may only differentiate human induced pluripotent stem cells (iPSCs) into immature cardiomyocytes. To achieve cardiomyocyte maturation, appropriate modulation of cellular microenvironment ... More