Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Catalog number: A1413202
Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Catalog number: A1413202
Catalog Number
A1413202
Unit Size
5 mL
Price (USD)
Price: 258.00
Your Price:
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
Price: 58.25
Your Price:
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
A14132025 mL
Price: 258.00
Your Price:
A14132011 mL
Price: 58.25
Your Price:
Product Overview
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Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco™ Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco™ primary cells and specialty media. See this and other angiogenesis assay protocols
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
12-18 mg/mL
Product Type
Basement Membrane Matrix
Sterility
Sterile
Endotoxin Level
Low
Purity or Quality Grade
Cell Culture Grade
Shipping Condition
Dry Ice
Product Line
Gibco™
Form
Frozen
Phenol Red Indicator
No Phenol Red
Shelf Life
36 months
Quantity
5 mL
Format
Tube(s)
Cell Type
Variety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC)
Without Additives
Phenol Red
Culture Type
Adherent Cell Culture
Species
Mouse
Classification
Reduced Growth Factor
Serum Level
Serum-Free

Contents & Storage

Store at -20°C.