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A-23187 Free Acid (Calcimycin)

Catalog number: A1493

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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The Ca2+ ionophore A-23187 is commonly used for in situ calibrations of fluorescent Ca2+ indicators, to equilibrate intracellular and extracellular Ca2+ concentrations and to permit Mn2+ to enter the cell to quench intracellular dye fluorescence. Although the intrinsic fluorescence of A-23197 is too high for use with UV-excitable calcium indicators like fura-2, indo-1 and quin-2 it is suitable for use with the visible light-excitable indicators, including fluo-4, fluo-3, Calcium Green and rhod-2. A-23187 can also be used to equilibrate extracelular and intrcellular Mg2+ concentrations, making it useful for calibrating Mg2+ indicators.
For Research Use Only. Not for use in diagnostic procedures.


Indicator Type: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Fluorometer, Flow Cytometer, Fluorescent Imager, Fluorescence Microscope
Cell Permeability: Cell-Permeant
Product Size: 10 mg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols