is commonly used for in situ calibrations of fluorescent Ca2+
indicators, to equilibrate intracellular and extracellular Ca2+
concentrations and to permit Mn2+
to enter the cell to quench intracellular dye fluorescence. Although the intrinsic fluorescence of A-23197 is too high for use with UV-excitable calcium indicators like fura-2, indo-1 and quin-2 it is suitable for use with the visible light-excitable indicators, including fluo-4, fluo-3, Calcium Green and rhod-2. A-23187 can also be used to equilibrate extracelular and intrcellular Mg2+
concentrations, making it useful for calibrating Mg2+
indicators.Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.