Informational:Taking your work to the clinic? Check out CTS™ Essential 6 »
Catalog Number
Unit Size
500 mL
Price (USD)
Price: 239.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1516401500 mL
Price: 239.00
Your Price:
Product Overview
Citations & References
Additional Information
Essential 6 Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs). In addition, Essential 6 Medium can be used as a base for custom media for the culture of PSCs. The formulation is based on a medium originally developed by Guokai Chen et. al. (1) in the laboratory of James Thomson and published as 'E6.' With only six essential components, Essential 6 Medium helps minimize variability. To complete your workflow with a matching reduced-variability PSC culture medium developed by the same lab, use Essential 8 Medium.

Essential 6 Medium enables:
Differentiation—does not contain bFGF, which inhibits differentiation
Reprogramming—does not contain TGFβ, which has a negative effect on reprogramming efficiency
Flexibility—provides a flexible format where the levels of TGFβ and bFGF can be adjusted and additional components can be added to match a given application

Unlike other media used in PSC culture, Essential 6 Medium does not contain bFGF or TGFβ. As such, Essential 6 Medium can support the formation of embryoid bodies. It has also been used as a base for the directed differentiation of various cell types in the endodermal, mesodermal, and ectodermal lineages (2), including motor neurons (3).

Essential 6 Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (4) and CytoTune (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.

Essential 6 Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFβ. This provides a basal medium that will maximize cell health and pluripotency while allowing levels of TGFβ and bFGF to be adjusted and additional components to be added to match a given application.

Commercialized in partnership with Cellular Dynamics International.

(1) Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 8(5):424-9.
(2) Lippmann ES, Estevez-Silva MC, Ashton RS. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells. 2014 32(4):1032-42.
(3) Lippmann ES, Williams CE, Ruhl DA, Estevez-Silva MC, Chapman ER, Coon JJ, Ashton RS. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm. Stem Cell Reports. 2015 4(4):632-44.
(4) Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009 324(5928):797-801.

For Research Use Only. Not for use in diagnostic procedures.


Cell Type
Pluripotent Stem Cells
Culture Type
Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Product Type
Stem Cell Media
Serum Level
With Additives
Phenol Red
Manufacturing Quality
cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product Line
Essential 6™
500 mL
Shipping Condition

Contents & Storage

Store at 2 to 8°C and protect from light.


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