Essential 6™ Medium

Catalog number: A1516401

Gibco™  Related applications: Clinical & Translational Research | Stem Cell Culture | Stem Cell Engineering & Reprogramming

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Description

Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs.

Essential 6™ Medium enables:

Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application

Reprogramming
Essential 6™ Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.

Differentiation
Essential 6™ Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6™ Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation.

Flexibility
Essential 6™ Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application.

Commercialized in partnership with Cellular Dynamics International.

References:
(1) Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods 2011 Apr 10.
(2) Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson J. (2009) Science 324:797-801.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (iPS - Induced Pluripotent Stem)
Form: Liquid
Species: Human
Quantity: 1 bottle(s)
Serum Level: Serum-Free
Culture Type: Feeder Cell-Free
Product Size: 500 mL
Classification: Xeno-Free
Volume (Metric): 500 ml

Contents & storage

1 × 500 ml bottle, Store at 2 to 8°C. Protect from light.

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Manuals & protocols

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