Alexa Fluor™ 647 Protein Labeling Kit, 1 kit - Citations
Alexa Fluor™ 647 Protein Labeling Kit, 1 kit - Citations
View additional product information for Antibody Labeling Kits for 1 mg - Citations (A10235, A10236, A10237, A10238, A20170, A10239, A20173, P30012, A20174, A20171, A20172, D20655, F10240, O10241, A10170)
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Abstract
CD8 T cell competition for dendritic cells in vivo is an early event in activation.
AuthorsWillis RA, Kappler JW, Marrack PC
JournalProc Natl Acad Sci U S A
PubMed ID16880405
'T cell responses against an antigen are often focused on a small fraction of potentially immunogenic determinants, a phenomenon known as immunodominance. Immunodominance can be established at several stages of antigen presentation, including antigen processing, binding of peptides to MHC, and competition between T cells for dendritic cells (DCs). The ... More
Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix.
'Studies in mouse, human, and chicken suggest that activation-induced deaminase (AID) is involved in three known processes leading to antibody diversification: somatic hypermutation, gene conversion, and class-switch recombination. Developing rabbit appendix provides a particularly good site for studying all three of these B cell maturation events. We report here successful ... More
Optimizing a waveguide-based sandwich immunoassay for tumor biomarkers: evaluating fluorescent labels and functional surfaces.
AuthorsMukundan H, Xie H, Anderson AS, Grace WK, Shively JE, Swanson BI,
JournalBioconjug Chem
PubMed ID19173652
'The sensor team at the Los Alamos National Laboratory has developed a waveguide-based optical biosensor for the detection of biomarkers associated with disease. We have previously demonstrated the application of this technology to the sensitive detection of carcinoembryonic antigen in serum and nipple aspirate fluid from breast cancer patients. In ... More
Allergic airways disease develops after an increase in allergen capture and processing in the airway mucosa.
Authorsvon Garnier C, Wikstrom ME, Zosky G, Turner DJ, Sly PD, Smith M, Thomas JA, Judd SR, Strickland DH, Holt PG, Stumbles PA,
JournalJ Immunol
PubMed ID17947647
'Airway mucosal dendritic cells (AMDC) and other airway APCs continuously sample inhaled Ags and regulate the nature of any resulting T cell-mediated immune response. Although immunity develops to harmful pathogens, tolerance arises to nonpathogenic Ags in healthy individuals. This homeostasis is thought to be disrupted in allergic respiratory disorders such ... More
VHH antibody targeting the chemokine receptor CX3CR1 inhibits progression of atherosclerosis.
AuthorsLow S, Wu H, Jerath K, Tibolla A, Fogal B, Conrad R, MacDougall M, Kerr S, Berger V, Dave R, Villalona J, Pantages L, Ahlberg J, Li H, Van Hoorick D, Ververken C, Broadwater J, Waterman A, Singh S, Kroe-Barrett R
Journal
PubMed ID31924119
'CX3CR1 has been identified as a highly attractive target for several therapeutic interventions. Despite this potential, no potent antagonists, either small molecule or monoclonal antibody, have been identified. Here we describe the lead finding and engineering approach that lead to the identification of BI 655088, a potent biotherapeutic antagonist to ... More
Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.
AuthorsIdoyaga J, Suda N, Suda K, Park CG, Steinman RM,
JournalProc Natl Acad Sci U S A
PubMed ID19168629
Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8alphaalpha in mice and is specialized to capture dying cells and process antigens for MHC class I ... More
Evidence for resonance optical trapping of individual fluorophore-labeled antibodies using single molecule fluorescence spectroscopy.
AuthorsLi H, Zhou D, Browne H, Klenerman D
JournalJ Am Chem Soc
PubMed ID16637638
We report single molecule fluorescence studies of the diffusion of individual multiple fluorophore-labeled antibodies in solution, which show that a trapping potential of about 3.6 k(B)T can be obtained at laser powers below 1 mW with resonant excitation. Individual antibodies can be trapped for up to 140 ms, and bound ... More
Fluorescence quantification for surface plasmon excitation.
AuthorsFeller BE, Kellis JT, Cascão-Pereira LG, Knoll W, Robertson CR, Frank CW,
JournalLangmuir
PubMed ID18844383
Surface plasmon resonance and surface plasmon fluorescence spectroscopy in combination have the potential to distinguish multicomponent surface processes. However, surface intensity variations from resonance angle shifts lead to a nonlinear response in the fluorescence intensity. We report a method to account for surface intensity variations using the experimentally measured relationship ... More
Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation.
AuthorsPhan TG, Green JA, Gray EE, Xu Y, Cyster JG,
JournalNat Immunol
PubMed ID19503106
Subcapsular sinus (SCS) macrophages capture antigens from lymph and present them intact for B cell encounter and follicular delivery. However, the properties of SCS macrophages are poorly defined. Here we show SCS macrophage development depended on lymphotoxin-alpha1beta2, and the cells had low lysosomal enzyme expression and retained opsonized antigens on ... More
A hyperspectral fluorescence system for 3D in vivo optical imaging.
AuthorsZavattini G, Vecchi S, Mitchell G, Weisser U, Leahy RM, Pichler BJ, Smith DJ, Cherry SR
JournalPhys Med Biol
PubMed ID16585843
In vivo optical instruments designed for small animal imaging generally measure the integrated light intensity across a broad band of wavelengths, or make measurements at a small number of selected wavelengths, and primarily use any spectral information to characterize and remove autofluorescence. We have developed a flexible hyperspectral imaging instrument ... More
Modulation of antigen presentation by autoreactive B cell clones specific for GAD65 from a type I diabetic patient.
AuthorsBanga JP, Moore JK, Duhindan N, Madec AM, van Endert PM, Orgiazzi J, Endl J
JournalClin Exp Immunol
PubMed ID14678267
We used a GAD65-specific human B-T cell line cognate system in vitro to investigate the modulation of GAD65 presentation by autoantibody, assessed in a proliferation assay. Generally, if the T cell determinant overlaps or resides within the antibody epitope, effects of presentation are blunted while if they are distant can ... More
A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.
AuthorsFriedman A, Perrimon N
JournalNature
PubMed ID17086199
Receptor tyrosine kinase (RTK) signalling through extracellular-signal-regulated kinases (ERKs) has pivotal roles during metazoan development, underlying processes as diverse as fate determination, differentiation, proliferation, survival, migration and growth. Abnormal RTK/ERK signalling has been extensively documented to contribute to developmental disorders and disease, most notably in oncogenic transformation by mutant RTKs ... More
Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide.
AuthorsOsterloh A, Kalinke U, Weiss S, Fleischer B, Breloer M
JournalJ Biol Chem
PubMed ID17164250
Activation of professional antigen-presenting cells (APC) is a crucial step in the initiation of an efficient immune response. In this study we show that Hsp60 mediates immune stimulation by different mechanisms, dependent and independent of lipopolysaccharide (LPS). We have demonstrated earlier that both, Hsp60 and LPS, increase antigen-specific interferon (IFN) ... More
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.
AuthorsVirant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Vojnovic I, Pisak-Lukáts B, Endesfelder U, Rothbauer U
JournalNat Commun
PubMed ID29500346
'Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb ... More
Periostin and CCN2 Scaffolds Promote the Wound Healing Response in the Skin of Diabetic Mice.
AuthorsElliott CG, Wang J, Walker JT, Michelsons S, Dunmore-Buyze J, Drangova M, Leask A, Hamilton DW
JournalTissue Eng Part A
PubMed ID30572781
'Nonhealing skin wounds remain a significant burden on health care systems, with diabetic patients 20 times as likely to undergo a lower extremity amputation due to impaired healing. Novel treatments that suppress the proinflammatory signature and induce the proliferative and remodeling phases are needed clinically. We demonstrate that the addition ... More
N-Linked Glycosylation Regulates CD22 Organization and Function.
AuthorsWasim L, Buhari FHM, Yoganathan M, Sicard T, Ereño-Orbea J, Julien JP, Treanor B
JournalFront Immunol
PubMed ID31019513
The organization and clustering of cell surface proteins plays a critical role in controlling receptor signaling; however, the biophysical mechanisms regulating these parameters are not well understood. Elucidating these mechanisms is highly significant to our understanding of immune function in health and disease, given the importance of B cell receptor ... More
Temporal Distribution Patterns of Alexa Fluor 647-Conjugated CeNPs in the Mouse Retina After a Single Intravitreal Injection.
AuthorsWong LL, Barkam S, Seal S, McGinnis JF
JournalAdv Exp Med Biol
PubMed ID31884600
Intravitreal (IVT) injection of ophthalmic therapeutics is the most widely used drug delivery route to the posterior segment of the eye. We employed this method to deliver our inorganic, catalytic antioxidant, cerium oxide nanoparticles (CeNPs), to rodent models of retinal degeneration. A single IVT of CeNPs delays disease progression. Even ... More
Genome editing in wheat microspores and haploid embryos mediated by delivery of ZFN proteins and cell-penetrating peptide complexes.
AuthorsBilichak A, Sastry-Dent L, Sriram S, Simpson M, Samuel P, Webb S, Jiang F, Eudes F
JournalPlant Biotechnol J
PubMed ID31729822
Recent advances in genome engineering technologies based on designed endonucleases (DE) allow specific and predictable alterations in plant genomes to generate value-added traits in crops of choice. The EXZACT Precision technology, based on zinc finger nucleases (ZFN), has been successfully used in the past for introduction of precise mutations and ... More