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Invitrogen
Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 546
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FIGURE: 1 / 12
Mouse IgG1 Cross-Adsorbed Secondary Antibody (A-21123) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 546 (Product # A-21123) was performed using SH-SY5Y (positive model) and T-47D (negative model) cells stained with Nestin Monoclonal Antibody (10C2), eBioscience™ (Product # 14-9843-80). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked... View More
Product Details
A-21123
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:10,000
Immunohistochemistry (Paraffin) (IHC (P))
1-10 µg/mL
Immunocytochemistry (ICC/IF)
1.5 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
IgG gamma 1
Conjugate
Excitation/Emission Max
561/572 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Amount
500 µg
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG1
Cross Adsorption
Prior to conjugation, antibody was solid phase adsorbed using mouse IgG2a, IgG2b and IgG3 immunosorbents to remove cross reactive antibody.
Antibody Form
Whole Antibody
Product Specific Information
Product will be shipped at Room Temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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