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Invitrogen
Golgin-97 Monoclonal Antibody (CDF4)
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FIGURE: 1 / 78
Golgin-97 Antibody (A-21270) in ICC/IF
Increased label specificity and resolution afforded by Image-iT® FX signal enhancer. Fixed and permeabilized MRC-5 human lung fibroblast cells were labeled with mouse anti-human Golgin-97 primary antibody (Product # A-21270), then visualized with either FITC-conjugated goat anti-mouse IgG (F2761, top row) or Alexa Fluor® 488 Goat Anti-Mouse IgG (Product # A-11001, bottom row). Cells on the right were treate... View More
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.jpg?time=20220826?time=20220826 "Golgin-97 Antibody (A-21270) in ICC/IF")
Product Details
A-21270
Applications
Tested Dilution
Publications
Product Specifications
Published species
C. elegans,
Chicken,
Dog,
Human,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CDF4
Immunogen
Human golgin-97.
Conjugate
Form
Lyophilized
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
Anti-human golgin-97 antibodies were originally isolated from the serum of a patient with the autoimmune disease known as Sjögren's syndrome. These antibodies recognize a 97 kDa protein called golgin-97, a member of the granin family of proteins and a peripheral membrane protein localized on the cytoplasmic face of the Golgi apparatus. Because the antibody recognizes a protein unique to the Golgi apparatus of most vertebrate species, it is useful for immunodetection and identification of the Golgi apparatus in cells.
To prepare a stock solution, reconstitute the antibody in 0.5-1 mL of phosphate-buffered saline, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2 mM sodium azide. For longer storage, divide the solution into single-use aliquots and freeze at -20°C.
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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