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Invitrogen
ATP Synthase beta Monoclonal Antibody (3D5AB1)
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FIGURE: 1 / 53
ATP Synthase beta Antibody (A-21351) in ICC/IF
Immunofluorescent analysis of ATP Synthase Subunit beta was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP Synthase Subunit beta (3D5AB1) Mouse Monoclonal Antibody (Product # A-21351) at 2 µg/mL in 0... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A-21351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
C. elegans,
Human,
Mouse,
Non-human primate,
Rat
Published species
C. elegans,
Fruit fly,
Human,
Insect,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3D5AB1
Immunogen
Human Heart Mitochondria.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity was judged by SDS-PAGE.
The epitope recognized by the anti-ATP synthase beta 3D5AB1 is in the region containing the active site of the beta-subunit and is centered approximately at amino acid residue 83. The complete amino acid sequence of the epitope is not known. See Chi SL, Wahl ML, Kenan DJ et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 67:4716-4724.
Target Information
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Atp5b; catalytic subunit; epididymis secretory protein Li 271; F-1-ATPase beta-subunit precursor; F1-ATPase beta-subunit; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit; unnamed protein product
Gene Aliases: ATP5B; ATPMB; ATPSB; DYT38; HEL-S-271; HUMOP2
UniProt ID: (Bovine) P00829, (Human) P06576, (Mouse) P56480, (Rat) P10719
Entrez Gene ID: (Bovine) 327675, (Human) 506, (Mouse) 11947, (Rat) 171374
angiogenesis
osteoblast differentiation
generation of precursor metabolites and energy
ATP biosynthetic process
ATP synthesis coupled proton transport
mitochondrial ATP synthesis coupled proton transport
positive regulation of blood vessel endothelial cell migration
ATP metabolic process
regulation of intracellular pH
hydrogen ion transmembrane transport
lipid metabolic process
receptor-mediated endocytosis
negative regulation of cell adhesion involved in substrate-bound cell migration
cellular response to interleukin-7
response to peptide
liver development
cold acclimation
response to manganese ion
cellular response to peptide
response to curcumin
response to 3,3',5-triiodo-L-thyronine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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