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Invitrogen
alpha Tubulin Monoclonal Antibody (236-10501), Biotin
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FIGURE: 1 / 6
alpha Tubulin Antibody (A21371) in WB
Western blot analysis of alpha-Tubulin was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3) and A431 (lane4) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. alpha-Tubulin was detected at ~ 52 kDa using alpha-Tubulin Biotin Conjugated ... View More
Product Details
A21371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Published species
C. elegans,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
236-10501
Immunogen
Bovine Alpha-Tubulin.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Storage and reconstitution: upon receipt, store desiccated at -20°C. To prepare a stock solution, reconstitute in PBS containing 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, aliquot and store at -20°C.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; TUBB; Tubulin alpha; tubulin alpha 6; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide; tubulin, beta polypeptide paralog
Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBB2B
UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Human) Q9BQE3, (Bovine) Q3ZCJ7, (Human) Q6PEY3, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) P68366, (Bovine) P81948, (Human) A6NHL2, (Bovine) Q6B856, (Human) A8K068, (Mouse) P68369, (Mouse) P05213, (Mouse) P68373, (Mouse) P68368, (Mouse) Q3UX10, (Mouse) Q9CWF2
Entrez Gene ID: (Bovine) 404053, (Human) 7846, (Bovine) 539882, (Human) 10376, (Human) 84790, (Bovine) 504244, (Human) 7278, (Human) 112714, (Bovine) 534900, (Human) 7277, (Bovine) 777775, (Bovine) 538631, (Human) 79861, (Bovine) 281555, (Human) 347733, (Mouse) 22142, (Mouse) 22143, (Mouse) 22146, (Mouse) 22145, (Mouse) 238463, (Mouse) 73710
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
protein domain specific binding
double-stranded RNA binding
ubiquitin protein ligase binding
molecular_function
enzyme binding
protein kinase binding
protein heterodimerization activity
nucleotide binding
tubulin
G2/M transition of mitotic cell cycle
cytoskeleton organization
microtubule-based process
cytoskeleton-dependent intracellular transport
cell division
microtubule cytoskeleton organization
cellular response to interleukin-4
biological_process
platelet degranulation
positive regulation of axon guidance
neuron migration
protein polymerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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