Western blot analysis of alpha-Tubulin was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3) and A431 (lane4) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. alpha-Tubulin was detected at ~ 52 kDa using alpha-Tubulin Biotin Conjugated ... View More

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

A21371

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1.0 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

236-10501

Immunogen

Bovine Alpha-Tubulin.

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

50 µg

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Ambient (domestic); Wet ice (international)

AB_10374524

Product Specific Information

Storage and reconstitution: upon receipt, store desiccated at -20°C. To prepare a stock solution, reconstitute in PBS containing 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, aliquot and store at -20°C.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 4 tubulin; alpha tubulin; alpha-1 tubulin; alpha-2; alpha-tubulin; alpha-tubulin 1; alpha-tubulin 2; alpha-tubulin 3; alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; alpha-tubulin 6; alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; Talpha1 alpha-tubulin; TUA; TUA1; TUA2; TUA3; TUBA1/3/4; TUBB; Tubulin alpha; tubulin alpha 6; tubulin alpha-1; tubulin alpha-1 chain; tubulin alpha-2 chain; tubulin alpha-3; tubulin alpha-3 chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; tubulin alpha-4 chain; tubulin alpha-6 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; tubulin H2-alpha; tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2B; tubulin, beta polypeptide; tubulin, beta polypeptide paralog; unnamed protein product

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; SPAX11; Tuba-1; TUBA1; TUBA2; TUBA3; TUBA3D; Tuba4; TUBA4A; TUBA6

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding microtubule binding identical protein binding macromolecular complex binding protein heterodimerization activity chaperone binding double-stranded RNA binding ubiquitin protein ligase binding enzyme binding protein kinase binding protein domain specific binding tubulin

Process(es)

microtubule cytoskeleton organization mitotic cell cycle neuron migration startle response protein folding 'de novo' protein folding intracellular protein transport microtubule-based process centrosome cycle smoothened signaling pathway memory locomotory behavior adult locomotory behavior visual learning response to mechanical stimulus glial cell differentiation gene expression dentate gyrus development cerebellar cortex morphogenesis hippocampus development pyramidal neuron differentiation cerebral cortex development neurogenesis neuron differentiation sperm motility adult behavior cytoskeleton-dependent intracellular transport response to tumor necrosis factor locomotory exploration behavior microtubule polymerization forebrain morphogenesis homeostasis of number of cells within a tissue regulation of synapse organization synapse organization protein stabilization cell division neuron apoptotic process motor behavior cellular response to calcium ion organelle transport along microtubule neuron projection arborization response to L-glutamate cytoskeleton organization positive regulation of axon guidance cellular response to interleukin-4 modulation of synaptic transmission embryonic brain development

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alpha Tubulin Monoclonal Antibody (236-10501), Biotin

alpha Tubulin Antibody (A21371) in WB