Nuclear localization and export signals of the human aryl hydrocarbon receptor.
AuthorsIkuta T, Eguchi H, Tachibana T, Yoneda Y, Kawajiri K
JournalJ Biol Chem
PubMed ID9446600
'The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that binds DNA in the form of a heterodimer with the Ahr nuclear translocator (hypoxia-inducible factor 1beta). We found in this study that Ahr contains both nuclear localization and export signals in the NH2-terminal region. A fusion protein composed of ... More
Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation.
AuthorsBasu S, Campagnola PJ
JournalBiomacromolecules
PubMed ID15003023
'We demonstrate micron scale control of bioactivity through the use of multiphoton excited photochemistry, where this technique has been used to cross-link three-dimensional matrixes of alkaline phosphatase, bovine serum albumin, and polyacrylamide and combinations therein. Using a fluorescence-based assay (ELF-97), the enzymatic activity has been studied using a Michaelis-Menten analysis, ... More
Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds. Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5.
AuthorsHryciw DH, Ekberg J, Ferguson C, Lee A, Wang D, Parton RG, Pollock CA, Yun CC, Poronnik P
JournalJ Biol Chem
PubMed ID16601121
'The constitutive reuptake of albumin from the glomerular filtrate by receptor-mediated endocytosis is a key function of the renal proximal tubules. Both the Cl- channel ClC-5 and the Na+-H+ exchanger isoform 3 are critical components of the macromolecular endocytic complex that is required for albumin uptake, and therefore the cell-surface ... More
Trichocyst membrane fate in living Paramecium primaurelia visualized by multimodal confocal imaging.
AuthorsRamoino P, Beltrame F, Diaspro A, Fato M
JournalEur J Cell Biol
PubMed ID9309393
'Trichocysts are secretory organelles located at the surface of several ciliates, docked at the plasma membrane. Their secretion is similar to other exocytic processes: the trichocyst membrane fuses with the plasma membrane, its content is released outside the cell, and the membrane is retrieved back into the cell. The fate ... More
High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast.
AuthorsAjo-Franklin CM, Kam L, Boxer SG
JournalProc Natl Acad Sci U S A
PubMed ID11717428
'Total internal reflection fluorescence microscopy is widely used to confine the excitation of a complex fluorescent sample very close to the material on which it is supported. By working with high refractive index solid supports, it is possible to confine even further the evanescent field, and by varying the angle ... More
The uptake of Texas Red-BSA in the excretory system of schistosomes and its colocalisation with ER60 promoter-induced GFP in transiently transformed adult males.
'The excretory system of schistosomes has focused some attention during the last years since accumulating evidence suggests that it plays an important role in the host-parasite interaction. Signalling molecules such as phosphatases, but also proteases have been localised in the excretory system. To some extent, however, localisation studies are limited ... More
Spatial and temporal imaging of gas phase protein and DNA produced by matrix-assisted laser desorption.
AuthorsHeise TW, Yeung ES
JournalAnal Chem
PubMed ID8135376
'The sensitivity of a transient imaging technique based on laser-excited fluorescence is shown to be within the levels required for studying matrix-assisted laser-desorption (MALD) of large biomolecules. Results show that film morphology, particularly film thickness, has a major influence on plume dynamics. Fluorescent labeling of protein and of DNA is ... More
Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.
AuthorsTitus JA, Haugland R, Sharrow SO, Segal DM
JournalJ Immunol Methods
PubMed ID6806389
The sulfonylchloride derivative of the red-emitting fluorophore, sulforhodamine 101, has been synthesized in order to provide a reagent for coupling to amino groups on proteins and other compounds, and it is now commercially available under the name 'Texas Red'. Texas Red conjugates of antibodies and other proteins have been prepared ... More
A study of the effect of surface damage on the uptake of Texas Red-BSA by schistosomula of Schistosoma mansoni.
AuthorsTan HH, Thornhill JA, Al-Adhami BH, Akhkha A, Kusel JR
JournalParasitology
PubMed ID12666882
In this paper we describe the effect of poly-L-lysines of different molecular weight on the schistosomula. In the control sample, the schistosomula of Schistosoma mansoni take up fluorescent Texas Red conjugated to bovine serum albumin (TxR-BSA) into the gut. Following slight damage by 24.0 kDa poly-L-lysine, a high proportion of ... More
Three-color fluorescence measurements on single cells excited at three laser wavelengths.
AuthorsSteinkamp JA, Stewart CC, Crissman HA
JournalCytometry
PubMed ID7056131
A three-laser flow cytometer for quantitative analysis and sorting of cells and microscopic particles has been developed and evaluated using argon- and krypton-ion lasers as excitation sources. Cells stained with three fluorescent dyes having different excitation spectra enter a flow chamber where they first pass through an electronic volume sensor ... More
Leakage responses to L-NAME differ with the fluorescent dye used to label albumin.
AuthorsRumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN
JournalAm J Physiol
PubMed ID9887048
Nitric oxide synthase (NOS) inhibitors have been reported to increase as well as to decrease microvascular transport of macromolecules in a variety of models. This study was performed to determine whether the influence of NOS inhibition on albumin leakage was dependent on the fluorescent dyes used to label albumin. Albumin ... More
beta-subunit of nuclear pore-targeting complex (importin-beta) can be exported from the nucleus in a Ran-independent manner.
AuthorsKose S, Imamoto N, Tachibana T, Yoshida M, Yoneda Y
JournalJ Biol Chem
PubMed ID9933584
The nuclear export of importin-alpha is mediated by CAS, which is related to importin-beta, whereas the mechanism for the export of importin-beta remains unclear. In this study, we demonstrate that the nuclear export of importin-beta is mediated by the nuclear pore complex-binding domain of this molecule. Insensitivity to leptomycin B ... More
Targeting of cell-surface beta-amyloid precursor protein to lysosomes: alternative processing into amyloid-bearing fragments.
AuthorsHaass C, Koo EH, Mellon A, Hung AY, Selkoe DJ
JournalNature
PubMed ID1608449
Progressive cerebral deposition of the amyloid beta-peptide is an early and invariant feature of Alzheimer's disease. The beta-peptide is released by proteolytic cleavages from the beta-amyloid precursor protein (beta APP), a membrane-spanning glycoprotein expressed in most mammalian cells. Normal secretion of beta APP involves a cleavage in the beta-peptide region, ... More
A prion protein cycles between the cell surface and an endocytic compartment in cultured neuroblastoma cells.
AuthorsShyng SL, Huber MT, Harris DA
JournalJ Biol Chem
PubMed ID8101844
The prion protein (PrPC) is a glycolipid-anchored, cell surface protein of unknown function, a posttranslationally modified isoform of which has been implicated in the pathogenesis of spongiform encephalopathies in man and animals. We report here the novel observation that chPrP, the chicken homologue of mammalian PrPC, constitutively cycles between the ... More
Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
AuthorsFrosst P, Guan T, Subauste C, Hahn K, Gerace L
JournalJ Cell Biol
PubMed ID11839768
Tpr is a coiled-coil protein found near the nucleoplasmic side of the pore complex. Since neither the precise localization of Tpr nor its functions are well defined, we generated antibodies to three regions of Tpr to clarify these issues. Using light and EM immunolocalization, we determined that mammalian Tpr is ... More
The characteristics and mechanisms of uptake of PLGA nanoparticles in rabbit conjunctival epithelial cell layers.
AuthorsQaddoumi MG, Ueda H, Yang J, Davda J, Labhasetwar V, Lee VH
JournalPharm Res
PubMed ID15139521
PURPOSE: To delineate the characteristics and mechanisms of uptake of biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles in primary cultured rabbit conjunctival epithelial cells (RCECs). METHODS: Poly(D,L-lactide-co-glycolide) nanoparticles (PLGA 50:50. 101 nm in diameter) containing 6-coumarin (as a fluorescent marker) were used. The effect of size was studied using various particle sizes (100 ... More
Measurement of plasma volume in rats with use of fluorescent-labeled albumin molecules.
AuthorsGillen CM, Takamata A, Mack GW, Nadel ER
JournalJ Appl Physiol
PubMed ID8175546
We describe a method for measuring plasma volume (PV) in small animals that allows small sample sizes but does not require the use of radioisotopes and thus is a convenient approach for making repeated measurements. Texas Red covalently bound to albumin (TR-A) was used in a typical indicator-dilution technique to ... More
Differential phototoxicity of fluorescent dye-labeled albumin conjugates.
AuthorsRumbaut RE, Sial AJ
JournalMicrocirculation
PubMed ID10501094
OBJECTIVE: Fluorescent dyes are commonly used as probes for assessment of macromolecular permeability. Despite numerous examples of light-dye induced toxicity in the microvasculature, little is known regarding the relative phototoxicity of commonly used fluorescent conjugates. We, therefore, compared the phototoxicity of four fluorescent conjugates of bovine serum albumin (BSA) available ... More
Diffusion of albumins in rat cortical slices and relevance to volume transmission.
AuthorsTao L, Nicholson C
JournalNeuroscience
PubMed ID8951877
The apparent diffusion coefficient, D*, was measured in rat cortical slices and compared to the free diffusion coefficient, D, for three negatively charged proteins, lactalbumin (mol. wt = 14,500), ovalbumin (45,000) and bovine serum albumin (66,000). The temporal evolution of the spatial distribution of albumin molecules labeled with the Texas ... More
The Mechanism of High-Output Cardiac Hypertrophy Arising From Potassium Channel Gain-of-Function in Cantú Syndrome.
Authors
JournalFunction (Oxf)
PubMed ID32865539
Micropatterning different cell types with microarray amplification of natural directional persistence.