SILAC DMEM Flex is Dulbecco’s Modified Eagle Medium (DMEM) without L-glutamine, L-lysine, and L-arginine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. DMEM is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of applications. Find the right modification using the media selector tool.
DMEM is unique from other media as it contains 4 times the concentrations of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. SILAC DMEM Flex has been further modified to not contain lysine, arginine, and glutamine. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), so requires a 5-10% CO₂ environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC DMEM Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.