SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

Catalog number: A2494301

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

Catalog number: A2494301
Catalog Number
Unit Size
500 mL
Price (USD)
Price: 103.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A2494301500 mL
Price: 103.00
Your Price:

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Product Overview
Citations & References
Additional Information
SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool.
This Advanced DMEM/F-12 is manufactured as follows:
• Non-essential amino acids• Glucose
• Sodium pyruvate• Phenol red
 • L-arginine
 • L-glutamine
 • L-lysine

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System
SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.


Cell Type
Mammalian Cells
Culture Type
Mammalian Cell Culture
Product Type
Dulbecco's Modified Eagle Medium
Serum Level
Reduced Serum
Shelf Life
12 Months
With Additives
Sodium Pyruvate, Non Essential Amino Acids
Without Additives
No Glucose, No Glutamine, No HEPES, No Phenol Red
Green Features
Sustainable packaging
Product Line
500 mL

Contents & Storage

Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.