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RevitaCell™ Supplement (100X) - FAQs

RevitaCell™ Supplement (100X) - FAQs

View additional product information for RevitaCell™ Supplement (100X) - FAQs (A2644501)

18 product FAQs found

Can RevitaCell Supplement be used in addition to a traditional ROCK Inhibitor?

No. Combination of RevitaCell Supplement with a traditional ROCK inhibitor will result in deleterious effects on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The RevitaCell Supplement in the PSC Cryopreservation Kit has a strong smell. Is this normal?

Yes, it is normal that the RevitaCell Supplement has a strong smell.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between the PSC Cryopreservation Kit and the CTS PSC Cyropreservation Kit?

The CTS PSC Cryopreservation Kit (Cat. No. A4239301) provides the same efficient recovery, consistency, and reduced variability as the PSC Cryopreservation Kit (Cat. No. A2644601). However, these two kits have different intended use statements, i.e., “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals” and “For Research Use Only”, respectively. Furthermore, the CTS PSC Cryopreservation Kit has full USP sterility testing info and a Drug Master file (DMF), whereas the sterility testing of the PSC Cryopreservation is via a modified USP method, and it does not have a DMF.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see in the flexible feeding schedules specified for StemFlex Medium that cells should be fed the day after passaging, however this is incompatible with my current workflow. Have you evaluated skipping the feed the day after passaging?

Yes. The main reason that the workflow states this is to accommodate ROCK inhibitor or RevitaCell Supplement that have been added during passaging. In such circumstances where ROCK inhibitor or RevitaCell Supplement are added, you should feed your cells within 18-24 hours post-passaging. However, if ROCK inhibitor or RevitaCell Supplement are not included at the time of passaging, then you may skip the day post-passaging.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to use ROCK inhibitor during single-cell passaging in StemFlex Medium?

The use of ROCK inhibitor is not required when culturing in StemFlex Medium on rhLaminin-521. However, supplementation with RevitaCell Supplement can provide additional support to PSCs during stressful transitions such as single-cell passaging.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the PSC Cryopreservation Kit?

The PSC cryopreservation kit contains xeno-free PSC Cryopreservation Medium, which is a ready-to-use solution for the cryopreservation of early passage pluripotent stem cells (PSCs), and Gibco Revitacell Supplement (100X), a chemically defined recovery supplement for use in the post-thaw culture medium. When used in combination, these reagents help minimize loss of cell viability, maximize post-thaw recovery, and minimize unwanted differentiation of PSCs. This kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs) to improve post-thaw cell viability and recovery.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Gibco RevitaCell Supplement help cell recovery after electroporation?

Yes, this has been tested with iPSCs. However, testing on primary cells has not been performed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Should cells be pre-treated with Gibco RevitaCell Supplement for 24 hours before cryopreservation?

No, such pre-treatment results in deleterious effects on the cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Gibco RevitaCell Supplement be used in addition to a traditional ROCK Inhibitor?

No. Combination of the Gibco RevitaCell Supplement with a traditional ROCK inhibitor results in deleterious effects on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Gibco RevitaCell Supplement be used in serum-supplemented media for culturing primary cells?

We have not tested this application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For feeder-free cultures, which types of feeder-free matrix has Gibco RevitaCell Supplement been shown to be compatible with?

Gibco RevitaCell Supplement been shown to be compatible with Gibco Geltrex matrix, Gibco Vitronectin, LN-521 Stem Cell Matrix, and iMatrix-511.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Gibco RevitaCell Supplement be used on iPSCs cultured on feeders or is this recommended only for feeder-free culture of iPSC?

Both types of cultures have been tested.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What species of primary cells has Gibco RevitaCell Supplement been used on?

We have only tested it with human species for the following cell types: corneal epithelial cells, keratinocytes, peripheral blood mononuclear cells, and rat cortical neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What species of PSCs has Gibco RevitaCell Supplement been used on?

We have only tested it with human PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I perform single cell passaging with Gibco Essential 8 Flex Medium?

Yes, Essential 8 Flex Medium is compatible with single cell passaging. Whenever you are performing single cell passaging, ensure that you use Gibco RevitaCell Supplement (Cat. No. A2644501) or another ROCK inhibitor.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use ROCK inhibitors with Gibco Essential 8 Flex Medium?

Yes, Essential 8 Flex Medium has been used with ROCK inhibitors. We suggest using Gibco RevitaCell Supplement (Cat. No. A2644501), which has been specifically designed to minimize the impact of stress on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add a ROCK inhibitor during passaging when culturing in Gibco Essential 8 Flex Medium?

As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.