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Bethyl Laboratories

Cytoskeletal Actin Polyclonal Antibody

View all (2) Cytoskeletal Actin antibodies

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Cytoskeletal Actin Polyclonal Antibody

Product Details

A300-491A-T

Applications
Tested Dilution
Publications

Western Blot (WB)

1:10,000-1:25,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000 to 1:5,000
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

N-terminus of human Beta-actin.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

10 µg

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat, X. tropicalis, Chicken, Sheep, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Golden hamster, Orangutan and Chimpanzee

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Atcb; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cytoskeletal gamma-actin; cytoskeleton actin; epididymis luminal protein 176; gamma actin; Gamma-actin; I(2)-actin; PS1TP5-binding protein 1; unnamed protein product

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Gene Aliases: ACT; ACTB; ACTG; ACTG1; Actl; Actx; AL023024; beta-actin; BKRNS; BNS; BRWS1; CSMH; DDS1; DFNA20; DFNA26; E430023M04Rik; E51; HEL-176; PS1TP5BP1; THC8

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UniProt ID: (Human) P60709, (Human) P63261, (Mouse) P60710, (Mouse) P63260

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Entrez Gene ID: (Human) 60, (Human) 71, (Mouse) 11461, (Mouse) 11465

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Function(s)
nucleotide binding structural constituent of cytoskeleton protein binding ATP binding hydrolase activity ATPase activity kinesin binding protein kinase binding nitric-oxide synthase regulator activity Tat protein binding nucleosomal DNA binding identical protein binding tau protein binding nitric-oxide synthase binding structural constituent of postsynaptic actin cytoskeleton transporter regulator activity profilin binding ubiquitin protein ligase binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
Process(es)
morphogenesis of a polarized epithelium chromatin remodeling regulation of transcription from RNA polymerase II promoter cytoskeleton organization establishment or maintenance of cell polarity axonogenesis positive regulation of cell proliferation substantia nigra development regulation of mitotic metaphase/anaphase transition adherens junction assembly maintenance of blood-brain barrier regulation of apoptotic process apical protein localization positive regulation of T cell differentiation negative regulation of cell differentiation positive regulation of cell differentiation positive regulation of myoblast differentiation positive regulation of transcription, DNA-templated cell motility regulation of norepinephrine uptake positive regulation of norepinephrine uptake regulation of cell cycle regulation of G0 to G1 transition platelet aggregation protein localization to adherens junction cellular response to cytochalasin B postsynaptic actin cytoskeleton organization regulation of transepithelial transport regulation of synaptic vesicle endocytosis positive regulation of stem cell population maintenance regulation of protein localization to plasma membrane positive regulation of double-strand break repair via homologous recombination regulation of G1/S transition of mitotic cell cycle regulation of double-strand break repair positive regulation of double-strand break repair regulation of nucleotide-excision repair angiogenesis positive regulation of gene expression positive regulation of cell migration sarcomere organization regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to interferon-gamma positive regulation of wound healing tight junction assembly protein localization to bicellular tight junction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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