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Bethyl Laboratories
RNF40 Polyclonal Antibody
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FIGURE: 1 / 1
RNF40 Antibody (A300-720A-T) in WB
Detection of human RNF40 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF40 antibody BL3315 (Product # A300-720A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate (B). RNF40 was also immunoprecipitated using rabbit anti-RNF40 antibodies BL3314 (Product #A300-719A) and BL3316 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minu... View More
Product Details
A300-720A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 600 and 650 of Human Ring Finger 40.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 95 kDa retinoblastoma protein binding protein; 95 kDa retinoblastoma-associated protein; BRE1 E3 ubiquitin ligase homolog B; BRE1-B; DKFZp686K191; E3 ubiquitin-protein ligase BRE1B; MGC13051; Rb-associated protein; RBP95; RING finger protein 40; ring finger protein 40, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase BRE1B; unnamed protein product
Gene Aliases: BRE1B; KIAA0661; RBP95; RNF40; STARING
UniProt ID: (Human) O75150
Entrez Gene ID: (Human) 9810
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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