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Bethyl Laboratories
RBM25/RED120 Polyclonal Antibody
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FIGURE: 1 / 1
RBM25/RED120 Antibody (A301-067A-T) in WB
Detection of human RBM25/RED120 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM25/RED120 antibody (Product # A301-067A) used for WB at 0.04 µg/mL (A) and 0.1 µg/mL (B) and used for IP at 3 µg/mg lysate. RBM25/RED120 was also immunoprecipitated by rabbit anti-RBM25/RED120 antibodies A301-068A and BL4629, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A... View More
Product Details
A301-067A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 150 and 200 of Human RNA binding motif protein 25 (Arg/Glu/Asp-rich protein, 120 kDa).
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arg/Glu/Asp-rich protein of 120 kDa; Arg/Glu/Asp-rich protein, 120 kDa; functional spliceosome-associated protein 94; Protein S164; RED120; rna-binding; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding region (RNP1, RRM) containing 7; RNA-binding region-containing protein 7; U1 small nuclear ribonucleoprotein 1SNRP homolog; unnamed protein product
Gene Aliases: fSAP94; NET52; RBM25; RED120; RNPC7; S164; Snu71
UniProt ID: (Human) P49756
Entrez Gene ID: (Human) 58517
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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