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Bethyl Laboratories
SMRT Polyclonal Antibody
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FIGURE: 1 / 1
SMRT Antibody (A301-147A) in WB
Detection of human SMRT by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMRT antibody (Product # A301-147A) used for WB at 0.04 µg/mL (A) and 1.0 µg/mL (B) and used for IP at 3 µg/mg lysate. SMRT was also immunoprecipitated by rabbit anti-SMRT antibody A301-148, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Product Details
A301-147A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1525 and 1575 of Human silencing mediator of retinoic acid and thyroid hormone receptor alpha.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTG repeat protein 26; N-CoR2; nuclear receptor corepressor; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid and thyroid hormone receptors extended isoform splice variant 1; transcriptional corepressor; SMAP270; SMRT; SMRTe; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TRAC
Gene Aliases: CTG26; N-CoR2; NCOR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618
Entrez Gene ID: (Human) 9612
DNA binding
chromatin binding
transcription corepressor activity
Notch binding
protein binding
enzyme activator activity
ligand-dependent nuclear receptor binding
glucocorticoid receptor binding
histone deacetylase binding
retinoic acid receptor binding
macromolecular complex binding
retinoid X receptor binding
negative regulation of transcription from RNA polymerase II promoter
lactation
regulation of cellular ketone metabolic process
cerebellum development
response to estradiol
estrous cycle
negative regulation of transcription, DNA-templated
negative regulation of androgen receptor signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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