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Bethyl Laboratories
DDX50 Polyclonal Antibody
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FIGURE: 1 / 1
DDX50 Antibody (A301-312A) in WB
Detection of human DDX50 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-DDX50 antibody (Product # A301-312A) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time 10 seconds.
Product Details
A301-312A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 687 and 737 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. DDX50 and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. DDX50 gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX50; DEAD (Asp-Glu-Ala-Asp) box polypeptide 50; DEAD box protein 50; DEAD-box helicase 50; Gu-beta; malignant cell derived RNA helicase; Nucleolar protein Gu2; RNA helicase II/Gu beta; unnamed protein product
Gene Aliases: DDX50; GU2; GUB; mcdrh; RH-II/GuB
UniProt ID: (Human) Q9BQ39
Entrez Gene ID: (Human) 79009
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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