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Bethyl Laboratories
SPINDLY/CCDC99 Polyclonal Antibody
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FIGURE: 1 / 1
SPINDLY/CCDC99 Antibody (A301-354A-T) in WB
Detection of human Spindly/CCDC99 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Spindly/CCDC99 antibody (Product # A301-354A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. Spindly/CCDC99 was also immunoprecipitated by rabbit anti-Spindly/CCDC99 antibody A301-355A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure tim... View More
Product Details
A301-354A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 475 and 525 of Human SPINDLY (coiled-coil domain containing 99).
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARG1; Arsenite-related gene 1 protein; Coiled-coil domain-containing protein 99; hSpindly; Protein Spindly; Rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A; Spindle apparatus coiled-coil domain-containing protein 1; unnamed protein product
Gene Aliases: CCDC99; SPDL1
UniProt ID: (Human) Q96EA4
Entrez Gene ID: (Human) 54908
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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