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          • Primary Antibodies ›
          • eIF3d Antibodies

          Bethyl Laboratories

          eIF3D/EIF3S7 Polyclonal Antibody

          View all (14) eIF3d antibodies

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          Cite eIF3D/EIF3S7 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          eIF3D/EIF3S7 Antibody in Western Blot (WB)
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          eIF3D/EIF3S7 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          eIF3D/EIF3S7 Antibody (A301-759A-T) in WB

          Detection of human eIF3D/EIF3S7 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3D/EIF3S7 antibody (Product # A301-759A) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          eIF3D/EIF3S7 Antibody in Western Blot (WB)
          eIF3D/EIF3S7 Polyclonal Antibody

          Product Details

          A301-759A-T

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 200 and 250 of human eukaryotic translation initiation factor 3, subunit D (eukaryotic translation initiation factor 3, subunit 7).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          TBS, pH 7.0 to 8.0, with 0.1% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine and Orangutan

          Target Information

          eIF3D is a protein belonging to the largest of the eukaryotic translation initiation factor family, eukaryotic translation initiation factor-3 (eIF3). eIF3 is a multiprotein complex composed of at least 13 different subunits. eIF3D binds to the 40S ribosomal subunit and thus helps in maintaining the 40S and 60S ribosomal subunits in a dissociated state. An important role of eIF3D is the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and promotes mRNA binding. eIF3D is the major RNA binding subunit of the eIF3 complex. It associates with the subunit p170 of eIF-3. Ubiquitous expression of eIF3D is seen in most of the tissues and it is phosphorylated upon DNA damage, likely by ATM or ATR.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: eIF-3-zeta; eIF3 p66; eIF3-p66; eIF3d; EIF3S7; Eukaryotic translation initiation factor 3 subunit 7; Eukaryotic translation initiation factor 3 subunit D; eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa; RP5-1119A7.12-003; translation initiation factor eIF3 p66 subunit; unnamed protein product

          View more View less

          Gene Aliases: eIF3-p66; eIF3-zeta; EIF3D; EIF3S7

          View more View less

          UniProt ID: (Human) O15371

          View more View less

          Entrez Gene ID: (Human) 8664

          View more View less

          Function(s)
          RNA binding translation initiation factor activity protein binding mRNA cap binding
          Process(es)
          formation of cytoplasmic translation initiation complex cytoplasmic translational initiation cap-dependent translational initiation translation translational initiation IRES-dependent viral translational initiation viral translational termination-reinitiation
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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