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Bethyl Laboratories
PKN2 Polyclonal Antibody
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FIGURE: 1 / 1
PKN2 Antibody (A302-443A-T) in WB
Detection of human PKN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PKN2 antibody (Product # A302-443A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. PKN2 was also immunoprecipitated by rabbit anti-PKN2 antibody A302-444A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Product Details
A302-443A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 200 and 250 of Human protein kinase N2.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
PKN2 (PRK2), a close relative of the PKCs, is a regulator of mitosis and exit from cytokinesis. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiolipin-activated protein kinase Pak2; PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2; RP5-905H16.1; Serine/threonine-protein kinase N2; unnamed protein product
Gene Aliases: Pak-2; PAK2; PKN2; PRK2; PRKCL2; PRO2042; STK7
UniProt ID: (Human) Q16513
Entrez Gene ID: (Human) 5586
nucleotide binding
RNA binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
small GTPase binding
histone deacetylase binding
cadherin binding
RNA polymerase binding
protein serine kinase activity
protein phosphorylation
apoptotic process
cell adhesion
signal transduction
epithelial cell migration
cell projection organization
positive regulation of cytokinesis
intracellular signal transduction
apical junction assembly
positive regulation of viral genome replication
positive regulation of mitotic cell cycle
cell division
vascular endothelial cell response to laminar fluid shear stress
regulation of cell motility
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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