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Bethyl Laboratories
SF3A3 Polyclonal Antibody
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FIGURE: 1 / 1
SF3A3 Antibody (A302-506A-T) in WB
Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody (Product # A302-506A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. SF3A3 was more efficiently immunoprecipitated by rabbit anti-SF3A3 antibody A302-507A, which recognizes a downstream epitope. Detection: Chemilumin... View More
Product Details
A302-506A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 75 and 125 of Human splicing factor 3a, subunit 3, 60kDa.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: pre-mRNA splicing factor SF3a (60kD); SAP 61; SF3a60; spliceosome associated protein 61; Spliceosome-associated protein 61; Splicing factor 3A subunit 3; splicing factor 3a, subunit 3, 60kD; splicing factor 3a, subunit 3, 60kDa; unnamed protein product
Gene Aliases: 4930512K19Rik; 60kDa; PRP9; PRPF9; SAP61; SF3A3; SF3a60
UniProt ID: (Human) Q12874, (Mouse) Q9D554
Entrez Gene ID: (Human) 10946, (Mouse) 75062
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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