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Bethyl Laboratories
PDE3A Polyclonal Antibody
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FIGURE: 1 / 1
PDE3A Antibody (A302-740A) in WB
Detection of human PDE3A by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3A antibody (Product # A302-740A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. PDE3A was also immunoprecipitated by rabbit anti-PDE3A antibody A302-741A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Product Details
A302-740A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 450 and 500 of human Phosphodiesterase 3A.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents (Meacci et al., 1992 [PubMed 1315035]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP phosphodiesterase, myocardial cGMP-inhibited; cGI-PDE; CGI-PDE A; cGMP-inhibited 3',5'-cyclic phosphodiesterase 3A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; cGMP-inhibited cAMP phosphodiesterase; Cyclic GMP-inhibited phosphodiesterase A; phosphodiesterase 3A, cGMP-inhibited
Gene Aliases: CGI-PDE; CGI-PDE A; CGI-PDE-A; HTNB; PDE3A
UniProt ID: (Human) Q14432
Entrez Gene ID: (Human) 5139
3',5'-cyclic-nucleotide phosphodiesterase activity
3',5'-cyclic-AMP phosphodiesterase activity
cGMP-inhibited cyclic-nucleotide phosphodiesterase activity
protein binding
phosphoric diester hydrolase activity
hydrolase activity
estrogen receptor activity
metal ion binding
3',5'-cyclic-GMP phosphodiesterase activity
estrogen binding
oocyte maturation
lipid metabolic process
signal transduction
G-protein coupled receptor signaling pathway
response to xenobiotic stimulus
cGMP-mediated signaling
intracellular steroid hormone receptor signaling pathway
regulation of meiotic nuclear division
negative regulation of apoptotic process
negative regulation of vascular permeability
positive regulation of vascular permeability
oocyte development
positive regulation of oocyte development
regulation of ribonuclease activity
cellular response to cGMP
cellular response to transforming growth factor beta stimulus
apoptotic signaling pathway
negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
negative regulation of cAMP/PKA signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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