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Bethyl Laboratories
GTF2B/TFIIB Polyclonal Antibody
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FIGURE: 1 / 1
GTF2B/TFIIB Antibody (A302-885A-T) in IP
Detection of human GTF2B/ TFIIB by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-GTF2B/ TFIIB antibody (Product # A302-885A) used for IP at 3 µg/mg lysate. GTF2B/ TFIIB was also immunoprecipitated by rabbit anti-TFIIB antibody A302-884A, which recognizes an upstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB, A302-884A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A302-885A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 256 and 316 of human General Transcription Factor IIB.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, X. laevis, Bovine and Orangutan
Target Information
Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: General transcription factor TFIIB; RNA polymerase II transcription factor IIB; RP11-82K18.1; S300-II; transcription factor; Transcription initiation factor IIB; unnamed protein product
Gene Aliases: GTF2B; TF2B; TFIIB
UniProt ID: (Human) Q00403
Entrez Gene ID: (Human) 2959
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II core binding
DNA binding
histone acetyltransferase activity
protein binding
zinc ion binding
obsolete general RNA polymerase II transcription factor activity
acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
TBP-class protein binding
metal ion binding
thyroid hormone receptor binding
peptide-lysine-N-acetyltransferase activity
DNA-binding transcription factor binding
promoter-specific chromatin binding
transcriptional start site selection at RNA polymerase II promoter
chromatin remodeling
DNA-templated transcription, initiation
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
protein acetylation
gene expression
viral transcription
RNA polymerase II transcriptional preinitiation complex assembly
meiotic sister chromatid cohesion
spindle assembly
chromosome organization
transcription preinitiation complex assembly
positive regulation of core promoter binding
RNA Polymerase II core complex assembly
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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