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Bethyl Laboratories
HSF1 Polyclonal Antibody
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FIGURE: 1 / 3
HSF1 Antibody (A303-176A-T) in IHC (P)
Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 (Product # A303-174A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (left), (Product # A303-175A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (center) and (Product # A303-176A) (Lot 1) used at a dilution of 1:1,000 (0.2 µg/mL) (right). Detection: DAB.
Product Details
A303-176A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 479 and 529 of human Heat Shock Transcription Factor 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSF1; HSTF 1; unnamed protein product
Gene Aliases: HSF1; HSTF1
UniProt ID: (Human) Q00613
Entrez Gene ID: (Human) 3297
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
heat shock protein binding
chromatin DNA binding
identical protein binding
sequence-specific DNA binding
protein heterodimerization activity
Hsp90 protein binding
translation elongation factor binding
STAT family protein binding
sequence-specific single stranded DNA binding
general transcription initiation factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
in utero embryonic development
embryonic placenta development
DNA repair
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
mRNA processing
defense response
cellular response to DNA damage stimulus
female meiotic division
spermatogenesis
response to nutrient
cell proliferation
response to heat
positive regulation of gene expression
negative regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
response to activity
negative regulation of protein complex assembly
response to estradiol
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
response to testosterone
cellular response to heat
cellular response to unfolded protein
cellular response to potassium ion
positive regulation of multicellular organism growth
response to amino acid
positive regulation of macrophage differentiation
positive regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
epithelial cell proliferation
negative regulation of epithelial cell proliferation
mRNA transport
embryonic process involved in female pregnancy
positive regulation of stress granule assembly
protein-containing complex assembly
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
cellular response to cadmium ion
cellular response to copper ion
cellular response to estradiol stimulus
cellular response to xenobiotic stimulus
cellular response to radiation
cellular response to gamma radiation
cellular response to diamide
negative regulation of inclusion body assembly
positive regulation of inclusion body assembly
positive regulation of cold-induced thermogenesis
regulation of cellular response to heat
response to peptide
positive regulation of apoptotic DNA fragmentation
cellular response to sodium arsenite
cellular response to angiotensin
cellular response to nitroglycerin
cellular response to L-glutamine
response to hypobaric hypoxia
response to psychosocial stress
negative regulation of double-strand break repair via nonhomologous end joining
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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