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Bethyl Laboratories
PARN Polyclonal Antibody
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FIGURE: 1 / 1
PARN Antibody (A303-561A) in WB
Detection of human PARN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PARN antibody (Product # A303-561A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. PARN was also immunoprecipitated by rabbit anti-PARN antibody A303-562A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1... View More
Product Details
A303-561A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 539 and 589 of human Poly(A)-Specific Ribonuclease (Deadenylation Nuclease).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deadenylating nuclease; Deadenylation nuclease; Poly(A)-specific ribonuclease PARN; Polyadenylate-specific ribonuclease; unnamed protein product
Gene Aliases: DAN; DKCB6; PARN; PFBMFT4
UniProt ID: (Human) O95453
Entrez Gene ID: (Human) 5073
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA poly(A) tail shortening
box H/ACA snoRNA 3'-end processing
RNA catabolic process
mRNA catabolic process
female gamete generation
RNA modification
miRNA catabolic process
positive regulation of macromolecule metabolic process
negative regulation of macromolecule metabolic process
positive regulation of telomere maintenance via telomerase
regulation of RNA stability
positive regulation of nucleobase-containing compound metabolic process
polyadenylation-dependent snoRNA 3'-end processing
telomerase RNA stabilization
lncRNA processing
regulation of telomerase RNA localization to Cajal body
priRNA 3'-end processing
siRNA 3'-end processing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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