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Bethyl Laboratories
PARP12 Polyclonal Antibody
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FIGURE: 1 / 2
PARP12 Antibody (A305-130A-T) in WB
Detection of human and mouse PARP12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARP12 antibody (Product # A305-130A) (lot A305-130A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
Product Details
A305-130A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 300 to 350 of human Poly [ADP-ribose] polymerase 12.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 12; ARTD12; PARP-12; Poly [ADP-ribose] polymerase 12; poly ADP-ribose polymerase 12; Protein mono-ADP-ribosyltransferase PARP12; unnamed protein product; Zinc finger CCCH domain-containing protein 1; zinc finger CCCH type domain containing 1
Gene Aliases: 9930021O16; AA409132; AA536654; ARTD12; MST109; MSTP109; PARP-12; PARP12; ZC3H1; ZC3HDC1
UniProt ID: (Human) Q9H0J9, (Mouse) Q8BZ20
Entrez Gene ID: (Human) 64761, (Mouse) 243771
RNA binding
NAD+ ADP-ribosyltransferase activity
zinc ion binding
transferase activity
transferase activity, transferring glycosyl groups
nucleotidyltransferase activity
metal ion binding
NAD+-protein-cysteine ADP-ribosyltransferase activity
NAD+-protein-aspartate ADP-ribosyltransferase activity
protein ADP-ribosylase activity
poly(A) RNA binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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