Alexa Fluor® 405 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. Alexa Fluor® 405 is a bright, violet dye with excitation ideally suited to the 405 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 405 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor® 405 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).
Detailed information about this AlexaFluor® cadaverine:
• Fluorophore label : Alexa Fluor® 405 dye • Reactive group: cadaverine • Reactivity: carboxylic acids, aldehydes, and ketones (and glutamine residues through an enzyme-catalyzed transamidation reaction) • Ex/Em of the conjugate: 399/422 nm • Extinction coefficient: 29,000 cm-1M-1 • Spectrally similar dyes: Cascade Blue • Molecular weight: 666.58
Cell Tracking and Tracing Applications Alexa Fluor® cadaverines make excellent fluorescent polar tracers because they are bright, small, and water soluble. Since they contain an aldehyde-fixable functional group, they can be fixed in cells by treatment with formaldehyde or glutaraldehyde. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent. Learn more about cell tracking and tracing.
Protein Labeling Applications Alexa Fluor® cadaverines can be used as reactive molecules for adding a fluorescent label to carboxylic acids using a coupling agent such as a carbodiimide; they do not spontaneously react with carboxylic acids in solution. They do, however, react spontaneously with the common amine-reactive functional groups, including succinimidyl esters and isothiocyanates. The amine-containing Alexa Fluor® cadaverines can also be used to label glutamine residues in some proteins and peptides via an enzyme-catalyzed transamidation reaction.
We’ll Make a Custom Conjugate for You If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
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For Research Use Only. Not for use in diagnostic procedures.
Carboxylic Acid, Ketone, Aldehyde
Alexa Fluor Dyes
Label or Dye:
Alexa Fluor™ 405
Contents & storage
Store in freezer (-5 to -30°C) and protect from light.