PSC Dopaminergic Neuron Differentiation Kit - FAQs

View additional product information for PSC Dopaminergic Neuron Differentiation Kit - FAQs (A3147701)

4 product FAQs found

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

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Is cell density in Floor Plate Specification medium critical for Dopaminergic neuron differentiation?

Yes, cell density should be optimized to attain full confluency at day 7 in the specification medium to have an efficient differentiation.

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Can Dopaminergic Neuron Maturation medium be used to differentiate neural stem cells?

We have tested neural stem cells (NSCs) isolated from fetal tissue or derived from pluripotent stem cells (PSCs), and have seen that both populations can benefit from maturation medium (DMEM/F12 + Dopaminergic Neuron Maturation Supplement) to have nicely spread homogenous neurons with reduced progenitor population. Matured neurons can be further maintained in neurobasal medium supplemented with Dopaminergic Neuron Maturation Supplement.

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Do you offer Dopaminergic Neuron differentiation service?

Yes. Please go here (https://www.thermofisher.com/us/en/home/life-science/stem-cell-research/stem-cell-services/stem-cell-differentiation-services.html).

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