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Sérum de veau fœtal, qualifié, Australie
Gibco™

Sérum de veau fœtal, qualifié, Australie

Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus
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RéférenceQuantitéFormatPureté ou qualité
A316100150 mLOne ShotQualifié
10099141500 mLFlaconQualifié
100991581 000 mLFlaconQualifié
A316100210 x 50 mLOne ShotQualifié
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Référence A3161001
Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Catégorie du sérum : Premium (Secure)
Origine : Australie.
Niveau d’endotoxine : ≤ 5 UE/ml.
Niveau d’hémoglobine : ≤ 30 mg/dl (généralement ≤ 25 mg/dl).
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Spécifications
DescriptionSérum de veau fœtal, qualifié, format One Shot™, Australie
Concentration en endotoxines≤5 UE/mL
Concentration d’hémoglobine≤ 30 mg/dl
Gamme de produitsOne Shot
Pureté ou qualitéQualifié
Conditions d’expéditionCongelé
EspècesBovins
AgeFœtal
Pays d’origineAustralie
FormeLiquide
FormatOne Shot
Type de produitSérum de veau fœtal
Quantité50 mL
Serum TreatmentStandard (filtration stérile)
StérilitéStérile
Unit SizeEach
Contenu et stockage
Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

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