Pierce™ Phosphatase Inhibitor Mini Tablets, 20 Tablets - FAQs

We have no data on long-term stability of the inhibitors at 4°C in aqueous solution. We generally recommend dissolving the tablets just before use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

As it is recommended to use a final inhibitor concentration of 2X - 3X for samples with a high phosphatase activity, it is indeed possible to prepare a solution at these concentrations. However, we would not recommend to go much higher than 3X.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

The Pierce Phosphatase Inhibitor Mini Tablets can be dissolved in the lysis reagent that is being used for protein extraction. We recommend dissolving each tablet in 10 mL of the lysis reagent to get a 1X final concentration. To dissolve completely, the solution may be vortexed or placed on a magnetic stir plate with a stir bar.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets are stable for at least one year when stored at 4 degrees C, but we only recommend preparing fresh solutions just before needed. If you are interested in liquid protease or phosphatase inhibitors, please use our inhibitor cocktails (https://www.thermofisher.com/search/results?query=protease%20inhibitor%20cocktails&focusarea=Search%20All).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are compatible with common protein assays such as BCA Protein Assay, 660 nm Protein Assay, Coomassie Plus (Bradford) Assay, and Detergent Compatible Bradford Assay.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Metalloproteases bind metal ions in their active sites. EDTA is a metalloprotease inhibitor that acts by chelating metal ions that are required by these proteases for activity. We recommend using the protease or phosphatase inhibitor tablet or mini tablet containing EDTA if any metalloprotease is likely to adversely affect the functioning of the target protein. However, it is important to avoid using EDTA when the downstream application involves metal chelation affinity purification or if the target protein requires metals ions for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitors are offered as mini tablets, with or without EDTA. Each mini tablet is sufficient for 10 mL of solution and is suitable for reagent preparation prior to protein extraction for cultured cells and tissues. In addition, the Pierce protease inhibitor tablets are offered with or without EDTA and each tablet is sufficient for 50 mL of solution.

Here are the catalog numbers:
-Pierce Protease Inhibitor Mini Tablets (Cat. No. A32953)
-Pierce Protease inhibitor Mini Tablets, EDTA-free (Cat. No. A32955)
-Pierce Phosphatase Inhibitor Mini Tablets (Cat. No. A32957)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets (Cat. No. A32959)
-Pierce Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free (Cat. No. A32961)
-Pierce Protease Inhibitor Tablets (Cat. No. A32963)
-Pierce Protease Inhibitor Tablets, EDTA-free (Cat. No. A32965)

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.