ExpiSf9 Cells are a non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.
ExpiSf9 Cells: • Enable consistent protein expression performance for over 20 passages after thaw • Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System • Achieve maximum cell densities of 2x107 cells per mL in ExpiSf CD Medium • Exhibit fast recovery post-thaw and doubling times of approximately 24 hours • Achieve high performance growth in multiple culture volumes from 4 mL in deep-well plates to >3 L in shake flasks
ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5 mL of cells at 1 x 107 viable cells per mL in ExpiSf CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf CD Medium. Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.
For Research Use Only. Not for use in diagnostic procedures.