Pierce™ Sulfo-SANPAH, No-Weigh™ Format, 10 x 1 mg - FAQs

View additional product information for Pierce™ Sulfo-SANPAH, No-Weigh™ Format - FAQs (A35395, 22589)

2 product FAQs found

Can you provide the shelf-life for Sulfo-SANPAH?

Sulfo-SANPAH is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the reactive groups on Sulfo-SANPAH and which groups do they react with?

Sulfo-SANPAH is a heterobifunctional crosslinker that has a sulfo-NHS ester and a photoactivatable nitrophenyl azide group. The sulfo-NHS ester reacts with primary amines in pH 7-9 buffers to form stable amide bonds. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.