Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System
. Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium
. They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent
and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.
• Suspension culture cells facilitate high-throughput screening or scale-up
• Higher viral production levels than other suspension HEK 293 cells
• Rapid cell growth and high culture viabilities
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107
viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.
The cells are typically grown in flasks on a shaker platform in a humidified, CO2
cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.
For Research Use Only. Not for use in diagnostic procedures.